ANTIBACTERIAL ACTIVITY OF DIFFERENT ETHANOLIC EXTRACT OF ALGERIAN PROPOLIS AGAINST Staphylococcus aureus

Main Article Content

ABDELLATIF BOUDRA
IDIR BENBELKACEM
MOHAMED AMINE AISSA

Abstract

The antimicrobial activity of propolis extracts is well documented. Nevertheless, the properties of the Algerian propolis are poorly investigated. This fact could be due to the diversity of the climate and the geographic areas from which propolis is usually harvested. The present work is a contribution to evaluate the antimicrobial susceptibility testing of propolis varieties with high total polyphenols and flavonoids contents, chosen beforehand from an analysis made of 10 propolis samples. The disk diffusion method was employed to determine the antistaphylococcal activity of three EEAP (ethanolic extract of Algerian propolis) samples, at different concentrations (5%, 10, 20 and 30%), against a reference bacterium strain of Staphylococcus aureus, the most redoubtable agent in osteomyelitis. Inhibitory zone diameters for the tested bacterium were 14.99 mm for EEAP at 5% concentration, 16.41 mm at 10%, 18.08 mm at 20% and 19.16 mm at 30%, respectively. Our in vitro study revealed that EEAP with high polyphenols and flavonoids contents exhibit a strong antibacterial activity, and it may be indicated as an alternative solution for the treatment of staphylococcal osteomyelitis.

Keywords:
Algerian propolis, Staphylococcus aureus, osteomyelitis, inhibition zone.

Article Details

How to Cite
BOUDRA, A., BENBELKACEM, I., & AISSA, M. A. (2020). ANTIBACTERIAL ACTIVITY OF DIFFERENT ETHANOLIC EXTRACT OF ALGERIAN PROPOLIS AGAINST Staphylococcus aureus. BIONATURE, 40(1), 4-8. Retrieved from https://globalpresshub.com/index.php/BN/article/view/817
Section
Original Research Article

References

Hatzenbuehler J, Pulling TJ. Diagnosis and management of osteomyelitis. Am. Fam. Physician. 2011;84(9):1027–1033.

Zimmerli W, Trampuz A, Ochsner PE. Prosthetic-joint infections. N. Engl. J. Med. 2004;352(1):95-97.
DOI: 10.1056/NEJM200501063520120

Rezende GPSR, Pimenta FC, Costa LRRS. Antimicrobial activity of two Brazilian commercial propolis extracts. Braz. J. Oral. Sci. 2006;5(16):967-970.
Available:https://doi.org/10.20396/bjos.v5i16.8641876

Lahouel M, Boutabet K, Kebsa W, Alyane M. Polyphenolic fractions of Algerian propolis reverses doxorubicin-induced acute renal oxidative stress. Afr. J. Pharm. Pharmacol. 2010;4(10):712-720.

Júnior AF, Balestrin EC, Betoni JEC, Orsi Rde O, Cunha MLRS, Montelli AC. Propolis: Anti-Staphylococcus aureus activity and synergism with antimicrobial drugs. Mem. Inst. Oswaldo. Cruz, Rio de Janeiro. 2005;100(5):563-565.

Niken P, Phalimahtussadiyah R, Zakiah U. Properties and flavonoids content in propolis of some extraction method of raw propolis. Int. J. Pharm. Sci. 2014;6(6):338-340.

Boudra A, Hamdi M, Amara K. Role of propolis in osteointegration enhancement in allograft autoclaved implanted in femur of dogs. J. Veterinar. Sci. Technolo. 2014;2(5): 1-3.
DOI: 10.4172/2157-7579.1000173

Ezgi A, Hasan Y. Evaluation of the effect of propolis on implant stability by resonance frequency analysis and removal torque test. Biomed. J Sci & Tech. Res. 2017;5(1):1-6.
DOI: 10.26717/BJSTR.2017.01.000477

Grange JM, Davey RW. Antibacterial properties of the propolis (bee glue). J. R. Soc. Med. 1990;83:159-160.

Parolia A, Thomas S, Kundabala M, Mohan M. Propolis and its potential uses in oral health. Int. J. Med. Med. Sci. 2010;2(7):210-215.

Boudra A, Benallou B, Meliani S, Hamdi M. Comparison of ethanolic extract yield, ph, polyphenols and flavonoids in Algerian propolis collected from various geographic regions, Bionature. 2019;39(1):1- 6.

Hendi NK, Naher HS, Al-Charrakh AH. In vitro antibacterial and antifungal activity of Iraqi propolis. J. Med. Plants Res. 2011;5(20):5058-5066.

Olson ME, Horswill AR. Staphylococcus aureus osteomyelitis: Bad to the bone. Cell Host & Microbe. 2013;13(6):629-631.
DOI: 10.1016/j.chom.2013.05.015

Nedji N, Loucif-Ayad W. Antimicrobial activity of Algerian propolis in foodborne pathogens and its quantitative chemical composition. Asian. Pac. J. Trop. Dis. 2014;4(6):433-437.
DOI: 10.1016/S2222-1808(14)60601-0

Taylor RSL, Manandhar NP, Hudson JB, Towers GHN. Antiviral activity of Nepales medicinal plants. J. Ethno. Aharmacol. 1996;52:156-163.
DOI: 10.1016/0378-8741(96)01409-2.

Hernández-Pérez M, López-García RE, Rabanal RM, Darias V, Arias A. Antimicrobial activity of Visnea mocanera leaf extracts. J. Ethnopharmacol. 1994;41(1-2):115-119.

Kim YH, Chung HJ. The effect of Korean propolis against foodborne pathogens and transmission electron microscopic examination. N. Biotechnol. 2011;28(6):713-718.
DOI: 10.1016/j.nbt.2010.12.006

Gülçin I, Bursal E, Sehitoğlu MH, Bilsel M, Gören AC. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem. Toxicol. 2010;48(8-9):2227-2238.
DOI: 10.1016/j.fct.2010.05.053