INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY A PENTACYCLIC TRITERPENES -RICH FRACTION FROM Tabebuia hypoleuca (C. Wright) Urb. LEAVES IN U-251 GLIOBLASTOMA CELLS

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Published: 2019-06-26

Page: 70-87


LUZ MARÍA SÁNCHEZ PERERA *

Department of Pharmaceutical Researching, National Center of Animal Health, CENSA, Apdo. 10, CP 32700, San José de las Lajas, Mayabeque, Cuba.

ADA IVIS REGALADO VELOZ

Department of Pharmaceutical Researching, National Center of Animal Health, CENSA, Apdo. 10, CP 32700, San José de las Lajas, Mayabeque, Cuba.

ANA LÚCIA TASCA GOIS RUIZ

Faculty of Pharmaceutical Science, University of Campinas, UNICAMP, Campinas, SP, Brazil.

GIOVANNA BARBARINI LONGATO

Laboratório de Pesquisa em Biologia Celular e Molecular de Tumores e Compostos Bioativos, Universidade São Francisco, Bragança Paulista/SP, Brazil.

GIOVANNA FRANCISCO FIORITO

Divisão de Farmacologia e Toxicologia, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), UNICAMP, Campinas, SP, Brazil.

SIRLENE VALÉRIO TINTI

Divisão de Farmacologia e Toxicologia, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), UNICAMP, Campinas, SP, Brazil.

MARY ANN FOGLIO

Faculty of Pharmaceutical Science, University of Campinas, UNICAMP, Campinas, SP, Brazil.

JOÃO ERNESTO DE CARVALHO

Faculty of Pharmaceutical Science, University of Campinas, UNICAMP, Campinas, SP, Brazil.

M. IQBAL CHOUDHARY

H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Science, ICCBS, University of Karachi, Pakistan.

*Author to whom correspondence should be addressed.


Abstract

Background: Tabebuia genera had been reported several pharmacological activities. Cuba has numerous endemic species in this genera, but only our group reported the anti-inflammatory, in vitro and in vivo antiproliferative properties from the specie Tabebuia hypoleuca (C. Wright) Urb. In vitro studies demonstrated that 786-0 (kidney), MCF-7 (breast) and glioma (U-251) were among the cancer cell lines most sensitive to ethyl acetate extract of leaves treatment.

Purpose: The aim of this study was to research the induction of cell cycle arrest and apoptosis by a pentacyclic triterpenes -rich fraction from Tabebuia hypoleuca leaves in U- 251 glioblastoma cells and identify the triterpene structures in this fraction.

Methods: A rich fraction of pentacyclic triterpenes were proceeded for the cell cycle arrest and apoptosis on glioblastoma cells using Annexin V and flow cytometry. The chemical composition was determinate by chromatography and spectroscopic methods.

Results:  Our results showed that a pentacyclic triterpenes –rich fraction had the ability to cause cell arrest in G2/M phase and induce apoptosis. The elucidation of the chemical compounds in this fraction revealed a mixing of oleanolic, ursolic and betulinic acids. This finding about chemical composition in the first report by the genera.

Conclusion: Therefore, this study showed the presence of different pentacyclic triterpenes in T. hypoleuca and it have promising anticancer activity against glioblastoma.

Keywords: Tabebuia hypoleuca, cell cycle, apoptosis, pentacyclic triterpenes.


How to Cite

PERERA, L. M. S., VELOZ, A. I. R., RUIZ, A. L. T. G., LONGATO, G. B., FIORITO, G. F., TINTI, S. V., FOGLIO, M. A., CARVALHO, J. E. D., & CHOUDHARY, M. I. (2019). INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY A PENTACYCLIC TRITERPENES -RICH FRACTION FROM Tabebuia hypoleuca (C. Wright) Urb. LEAVES IN U-251 GLIOBLASTOMA CELLS. BIONATURE, 39(2), 70–87. Retrieved from https://globalpresshub.com/index.php/BN/article/view/756

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