EMBRYOGENESIS AND PLANT REGENERATION FROM TISSUE CULTURE OF SEHIMA NERVOSUM (ROTTL.) STAPF

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Published: 1995-01-06

Page: 27-30


S. D. PUROHIT *

Plant Biotechnology Laboratory, Department of Botany, M. L. Sukhadia University, Udaipur-313 001 (India)

GOTAM KUKDA *

Plant Biotechnology Laboratory, Department of Botany, M. L. Sukhadia University, Udaipur-313 001 (India)

*Author to whom correspondence should be addressed.


Abstract

A white, compact,  hard,  nodular embryogenic callus was obtained from mature caryopses of Sehima nervosum cultured on Murashige and Skoog's (1962) medium supplemented with 3.0 mgl-1 2,4-dichlorophenoxyacetic acid. Such embryogenic cultures were maintained on MS medium containing 2.0 mgl-1 2,4-D and 100 mgl-1 ascorbic acid. Embryogenic cultures organized into well defined grass embryos when transferred to MS medium containing 1.0 mg-1 2,4-D under dark conditions. These embryos when transferred to auxin free MS medium and kept in light developed into complete plantiets.  Out of 1000 embryos transferred for germination, 980 (Ca 98%) produced complete plantlets. Young plantlets were successfully transplanted to pots and grown to maturity in greenhouse. All the plants were green except ten albino.

Keywords: Sehimanervosum, Regeneration, Plantlet, Somatic Embryogenesis, Grass


How to Cite

PUROHIT, S. D., & KUKDA, G. (1995). EMBRYOGENESIS AND PLANT REGENERATION FROM TISSUE CULTURE OF SEHIMA NERVOSUM (ROTTL.) STAPF. BIONATURE, 15(1), 27–30. Retrieved from https://globalpresshub.com/index.php/BN/article/view/630

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