CALLUS FORMATION FROM PROTOPLAST CULTURES OF ALLIUM WAKEGI

Full Article - PDF

Published: 1994-01-02

Page: 23-28


BONG-BO SEO *

Department of Biology, Kyungpook National University, Taegu 702-701, Korea.

SUN-HEE LEE *

Department of Biology, Kyungpook National University, Taegu 702-701, Korea.

A. S. NAIR *

Department of Biology, Kyungpook National University, Taegu 702-701, Korea.

*Author to whom correspondence should be addressed.


Abstract

Protoplasts were enzymatically isolated from leaves obtained from bulb cultures of amphidiploid A. wakegi, and protoplasts were inoculated in KM8P liquid medium containing 0.1M Mannitol, 0.4m glucose. 0.4mg/12,4-D, 2 mg/1NAA and 1 mg/1BAP, and incubated in dark. Colony formation was observed within two weeks of inoculation, and developed into mini-callus after one month. The minicallus obtained were transferred to MS medium containing 3% sucrose, 0.4 mg/1 2,4-D, 2 mg/1 NAA. 1 mg/1 BAP, and 0.8% agarose, and developed into friable callus.

Keywords: Allium, protoplast culture, Callus formation


How to Cite

SEO, B.-B., LEE, S.-H., & NAIR, A. S. (1994). CALLUS FORMATION FROM PROTOPLAST CULTURES OF ALLIUM WAKEGI. BIONATURE, 14(1), 23–28. Retrieved from https://globalpresshub.com/index.php/BN/article/view/604

Downloads

Download data is not yet available.