HPLC ANALYSIS REVEALS PRESENCE OF ALKALOID IN LEAF AND SEED EXTRACTS OF TRICHODESMA INDICUM (L.) R. BR
NEELAMBRA VERMA *
School of Life Sciences,*SoS in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C. G. India
V. KOCHE *
School of Life Sciences,*SoS in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C. G. India
K. L. TIWARI *
School of Life Sciences,*SoS in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C. G. India
S. K. MISHRA *
School of Life Sciences,*SoS in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C. G. India
*Author to whom correspondence should be addressed.
Abstract
Alkaloid contents of leaf, flower and seed samples of Trichodesma indicum were extracted using five different methods. Harborne's method produced maximum amount of alkaloid residues from leaf, flower and seed samples. The color test, TLC and HPLC confirmed the presence of alkaloid in leaf and seeds but not in flowers.
Keywords: Alkaloid, Trichodesma indicum, HPLC, TLC.