MICROPROPAGATION OF MEDICINAL PLANTS II. WEDELIA CHINENSIS (OSBECK) MERR.

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Published: 2010-02-10

Page: 139-145


SUSANTA KUMAR MAITY *

Department of Botany, Chandernagore Govt. College, Chandernagore, Hooghly, West Bengal

BRIJ KUMAR TIWARI *

Department of Botany, Chandernagore Govt. College, Chandernagore, Hooghly, West Bengal

ANADI KUMAR KUNDU *

Department of Botany, Chandernagore Govt. College, Chandernagore, Hooghly, West Bengal

*Author to whom correspondence should be addressed.


Abstract

Methods for in vitro micropropagation of Wedelia chinensis by indirect organogenesis via callus were investigated by optimizing the concentrations and combinations of different phytohormones in MS medium. Callus was induced from the leaf explants in MS medium containing BAP and NAA. Callus proliferation with development of associated nodular structures was achieved on subculturing in MS medium fortified with BAP and L-glutamine. Caulogenesis was recorded after the subculture of the nodular light green friable calli on MS basal medium in 2 weeks time. Rhizogenesis was observed 7 days after reinoculation of the shoots in MS basal medium.
No detectable differences were recorded between the donor and in vitro generated plants with regard to biochemical and cytological parameters.

Keywords: Medicinal plant, Wedelia,, Micropropagation.


How to Cite

MAITY, S. K., TIWARI, B. K., & KUNDU, A. K. (2010). MICROPROPAGATION OF MEDICINAL PLANTS II. WEDELIA CHINENSIS (OSBECK) MERR. BIONATURE, 30(2), 139–145. Retrieved from https://globalpresshub.com/index.php/BN/article/view/508

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