VARIABILITY IN SEED PROTEINS OF TRITICALE
P. SHRIVASTAVA *
Department of Genetics, Barkatullah University, Bhopal 462 026
N. C. SHARMA *
Department of Genetics, Barkatullah University, Bhopal 462 026
*Author to whom correspondence should be addressed.
Abstract
Seeds often hexaploid triticale strains namely UC-15, UC-48, UC-56, UC-40, UC-43, UC-63, UC-68, T-40, 6T-392 and DTS-630 were crushed and macerated with cold extraction buffer. The resulting solution was filtered over muslin cloth and centrifuged. The supernatant was subjected to electrophoresis on polyacrylamide gel column as described by Davis (1964), 0.1% amido black was used for staining the seed proteins and 7% acetic acid was used for destaining. The triticale strains exhibited different protein bands varying in number from four to ten. The Rf values ranged from 0.10 to 0.90. Some of the bands were thicker than the others. The types of storage seed proteins may be, thus, further resolved using 2-D electrophoresis. The present results, however, suggest that there exists variability for such proteins in triticales which can be made use of in triticale improvement and varietal identification.
Keywords: Triticale, Seed Proteins, Electrophoresis