IN-VITRO REGENERATION AND CALLUS FORMATION FROM LEAVES OF A MEDICINAL PLANT, “SPONDIAS MANGIFERA” (L.)

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Published: 2008-02-03

Page: 79-83


INDU KUMARI *

Department of Botany, Ranchi University, Ranchi,-834008

R. K. PANDEY *

Department of Botany, Ranchi University, Ranchi,-834008

*Author to whom correspondence should be addressed.


Abstract

Callus proliferation was studied on leaf of explant of Spondias mangifera L. cultured on Murashige and Skoog's medium (MS) supplemented with auxin (2, 4D, IBA, IAA and NAA) and cytokinin (BAP) alone or in combination. Most suitable medium noted for shoot formation from  leaves was BAP (2.5 ppm) in MS medium. Shoot developed was rooted the best in MS medium with 0.5 ppm  NAA.  Culture kept under various conditions of light and temperature to find the suitable physical condition for the best growth.

Keywords: Spondias mangifera L., plant tissue culture, plantlets from leaves


How to Cite

KUMARI, I., & PANDEY, R. K. (2008). IN-VITRO REGENERATION AND CALLUS FORMATION FROM LEAVES OF A MEDICINAL PLANT, “SPONDIAS MANGIFERA” (L.). BIONATURE, 28(2), 79–83. Retrieved from https://globalpresshub.com/index.php/BN/article/view/391

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