BOAR SPERM MEMBRANE INTEGRITY TEST FOLLOWING PRESERVATION IN LIQUID STATE
B. C. DAS *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
K. M. BUJARBARUAH *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
S. SANYAL *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
P. K. DAS *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
P. R. GHOSE *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
S. NASKAR *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
M. H. KHAN *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
A. B. DAS *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
S. BANDOPADHYAY *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
R. K. BORDOLOI *
Department of Veterinary Physiology, WBUA&FS, 37 K. B. Sarani, Kolkata-37
*Author to whom correspondence should be addressed.
Abstract
Three each of Hampshire and cross bred boar (Hampshire X Khasi local) have been taken for this study. Immediately after collection of semen, dilution was made (1:3) in BTS extender added with ascorbic acid as antioxidant and stored at 24°C and evaluated for Hypo-Osmotic sperm swelling (HOSST) for every stipulated hours viz. 0, 24, 48 and 72 hours. For Hampshire the values obtained were 55.33 ±0.16, 50.11 ± 0.16, 44.11 ± 0.16 and 41.22 ± 0.16% at 0, 24, 48 and 72 hours respectively and that for Cross. bred were 52.45+0.19, 46.95+0.19, 40.01 + 0.19 and 39.33±0.19%. There was significance difference (p<0.05) up to 48 hours and between breed. However within breed difference was not much prominence.
Keywords: Boar, spermatozoa,, preservation, HOSST