EFFECT OF ADJUVANT ON IN VITRO SHOOT PRODUCTION OF AZADIRACHTA INDICA

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Published: 2004-02-03

Page: 73-77


AFAQUE QURAISHI *

Plant Tissue Culture Laboratory, School of Life science, Pt. Ravishankar Shukla University, Raipur (C. G.) 492010

K. BAGH *

Plant Tissue Culture Laboratory, School of Life science, Pt. Ravishankar Shukla University, Raipur (C. G.) 492010

VIJAYA KOCHE *

Plant Tissue Culture Laboratory, School of Life science, Pt. Ravishankar Shukla University, Raipur (C. G.) 492010

S. K. MISHRA *

Plant Tissue Culture Laboratory, School of Life science, Pt. Ravishankar Shukla University, Raipur (C. G.) 492010

*Author to whom correspondence should be addressed.


Abstract

Different adjuvant showed variation in shoot proliferation rate during in vitro propagation of Azadirachta indica (Neem). Shoot proliferation is a crucial stage of in vitro propagation for commercial viability of the technique. Maximum shoot proliferation occurred from nodal explants of 1-year-old plants placed on Driver & Kuniyuki medium (1984) containing 0.22 µM 6-benzyladenine and 500 mg/l  polyvinyl  pyrrollidone.  It is estimated that, using this protocol,  more than 15,000 shoots can be produced within 9 months from a single node, of 1-year-old plant of Neem.

Keywords: Micropropagation, PVP, Azadirachta indica.


How to Cite

QURAISHI, A., BAGH, K., KOCHE, V., & MISHRA, S. K. (2004). EFFECT OF ADJUVANT ON IN VITRO SHOOT PRODUCTION OF AZADIRACHTA INDICA. BIONATURE, 24(2), 73–77. Retrieved from https://globalpresshub.com/index.php/BN/article/view/202

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