In vitro Propagation of Ocimum Sanctum Linn by Using Growth Hormone Shoot Induction

Kajal *

Department of Biotechnology, Choudhary Devilal University, Sirsa, India.

*Author to whom correspondence should be addressed.


The majority of Indian homes include tulsi, often known as holy basil (Ocimum sanctum L). Although it is regarded as a spiritual plant, physiologically speaking, it is one of the most readily available antibiotics. Tulsi is the most significant herb in Ayurveda, and current research is confirming its health advantages. It is also one of the plants that are utilised widely in Ayurvedic treatments.It relieves physical, physiological, metabolic, and psychological stress due to its special mix of pharmacological activity. A substrate for the quick development and multiplication of commercially significant plants is provided by plant tissue culture. Determining the optimal explants type and medium conditions for large-scale in vitro Tulsi shoot induction is the aim of the current effort. In the current investigation, the nodal segment and shoot tips were employed as explants. The nodal segment reacted well with a frequency rate of about 90% on all MS media utilized in the current investigation, including media with BAP and media with different combinations of BAP and IAA. It was seen that one or more shoots were emerging from the explants' nodal area after 10 days of culture. In this work, we examined the effects of various PGR combinations and dosages on the in vitro micro propagation of Tulsi, a fragrant and therapeutic plant (Ocmium sanctum L.). Three distinct PGRs were utilized, namely 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), and indole-3-acetic acid (IAA). The optimal medium for inducing and multiplying shoots was found to be Murashige and Skoog (MS) medium supplemented with 0.25 mg/l BAP and 0.1 mg/l NAA. The MS medium exhibits average shoot formation with 0.025 mg/l IAA and 0.1 mg/l BAP. Our results demonstrate that Tulsi may be successfully micro-propagated in vitro with the appropriate PGR.

Keywords: Vitaro Tulsi, ocmium sanctum, BAP and IAA, propagation

How to Cite

Kajal. (2024). In vitro Propagation of Ocimum Sanctum Linn by Using Growth Hormone Shoot Induction. BIONATURE, 44(1), 1–10.


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Agrawal D. Role of Different Species of Ocimum in Indian Culture and Physical Health. Think India Journal. 2019;22(8): 226-232.

Bhattacharyya P, Bishayee A. Ocimum sanctum Linn. (Tulsi): an ethnomedicinal plant for the prevention and treatment of cancer. Anti-cancer drugs. 2013;24(7): 659-666

Bhumarkar R, Mahajan G, & Kumar A. Doubling Farmers Income and Attaining Resilience in Agriculture through Crop Diversification; 2021.

Cardoso JC, Sheng Gerald LT, Teixeira da Silva JA. Micropropagation in the twenty-first century. Plant cell culture protocols. 2018;17-46.

Debnath SC, Arigundam U. In vitro propagation strategies of medicinally important berry crop, lingonberry (Vaccinium vitis-idaea L.). Agronomy. 2020;10(5):744.

Espinosa-Leal CA, Puente-Garza CA, García-Lara S. In vitro plant tissue culture: means for production of biological active compounds. Planta. 2018;248:1-18.

Jakovljević D, Stanković M, Warchoł M, Skrzypek E. Basil (Ocimum L.) cell and organ culture for the secondary metabolites production: A review. Plant Cell, Tissue and Organ Culture (PCTOC). 2022;149(1-2):61-79.

Kishan AS, Paul M, Reddy J. In vitro micropropagation of Ocimum sanctum L.; 2022.

Vijayan K, Raju PJ, Tikader A, Saratchnadra B. Biotechnology of mulberry (Morus L.)-A review. Emirates Journal of Food and Agriculture. 2014;26(6):472.

Weber E. Invasive plant species of the world: a reference guide to environmental weeds. Cabi; 2017.

Gulati D, Priyanka MP, Nidhi I. In vitro studies of the Ocimum sanctum: Tulsi, Medicinal herb. American Journal of Partech Research. 2015;5(6).

Hussain A, Qarshi IA, Nazir H, Ullah I. Plant tissue culture: current status and opportunities. Recent advances in plant in vitro culture. 2012;6(10):1-28.

Isah T, Umar S, Mujib A, Sharma MP, Rajasekharan PE, Zafar N, Frukh A. Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield. Plant Cell, Tissue andOrgan Culture (PCTOC). 2018;132:239-265.

Agarwal R, Mishra P, Singh R. Evaluating the impact of religious icons and symbols on consumer’s brand evaluation: Context of Hindu religion. Journal of Advertising. 2021;50(4):372-390.

Cardoso JC, Oliveira ME, Cardoso FDC. Advances and challenges on the in vitro production of secondary metabolites from medicinal plants. Horticultura Brasileira. 2019;37:124-132.