Malaria Vaccines Targeting Pfs25 Antigen in Parasite Mosquito Stages to Block Transmission


Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar 90224, Indonesia.

Rif’at Hanifah

Undergraduate Program, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia.

Yenni Yusuf *

Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia.

*Author to whom correspondence should be addressed.


Transmission of malaria involves Anopheles mosquitoes as the vectors of the Plasmodium parasite. The malaria eradication program includes the distribution of insecticide-treated bednets. However, the Anopheles mosquitoes have developed insecticide resistance hence it is necessary to find other modalities to eradicate the disease. Because the parasites undergo several stages in the mosquito midgut before developing into infective stages that migrate to the salivary gland, it is of interest to target the mosquito stages in vaccination to block the transmission. Human vaccination with the antigen of the mosquito stages may induce the production of specific antibodies against the stages that might be transferred into the mosquito during blood meal, binding with the antigen of the mosquito stages in the midgut and subsequently disrupting the development of the parasite. Such a vaccine is called a transmission-blocking vaccine (TBV). An effective TBV should induce a  high titer of antibody for a long time. One of the leading antigens is Pfs25. Here, we review the update of TBV development targeting Pfs25, advances in the transmission-blocking assay, and challenges in the development of TBVs.

Keywords: TBV, Pfs25, malaria, plasmodium, mosquito stages, TBA

How to Cite

Hartono, Hanifah, R., & Yusuf, Y. (2024). Malaria Vaccines Targeting Pfs25 Antigen in Parasite Mosquito Stages to Block Transmission. BIONATURE, 44(2), 11–19.


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WHO. World malaria report; 2023, 30 November 2023. Retrieved 18 June 2024, from


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