Effect of Humic Acid and Powders of Some Plants Producing Allelopathic Compounds on Soil Properties and Productivity of Faba Bean

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Atef Fathy Ahmed
Adel Ahmed Fakkar
Abd Elaal Mohamed Abd-El- Kareem


This investigation was conducted at Shandaweel Agricultural Research Station Sohag Governorate during two successive growing seasons 2015/16 and 2016/17 to study the effect of using humic acid (200 mg kg-1 soil), powder of some plants for producing allelopathic compounds (Powder at rate 10 gm kg-1 soil of Datura stramonium, Euphorbia geniculata, Trifolium, alexandrinum) on soil properties and some physiological aspects in faba bean plants in addition to faba bean yield and broomrape retarding. The results demonstrated that, for all used treatments, a decrease in the bulk density and increase in total porosity, soil infiltration rate and hydraulic conductivity due to the increases in total porosity of the soil. Water holding capacity, field capacity and wilting point also increased, and this improved the hydro-physical properties of the soil. Chemical properties of the soil represented in pH were slightly lowered, while noticeably increased in cation exchange capacity, organic matter and available macronutrients content in the treated soils. Faba bean yield represented in number of pods, weight of pods, seeds weight (g) all / plant and 100-seed weight and seed yield (ardeb /fed) were increased compared to control. Photosynthetic pigments, proteins and carbohydrates contents in shoots and roots were higher in plants grown in the treated soil compared with untreated, while, total free amino acids and proline contents in shoots and roots. The analysis of allelopathic plants powder, sindicated that these plants contains some acids and phenolic compounds in there shoots such as Vanillic, ferulic, syrungic, p-coumaric, p-hydroxypenzoic, caffic and protocatoic acids.

Humic acid, allelopathy, soil physical and chemical properties, broomrape, faba bean.

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How to Cite
Ahmed, A. F., Fakkar, A. A., & Kareem, A. E. M. A.-E.-. (2020). Effect of Humic Acid and Powders of Some Plants Producing Allelopathic Compounds on Soil Properties and Productivity of Faba Bean. Asian Journal of Research and Review in Agriculture, 2(1), 52-69. Retrieved from https://globalpresshub.com/index.php/AJRRA/article/view/880
Original Research Article


Kononova MM. Soil organic matter. Its role in soil formation and in soil fertility. Pergamon Press, Oxford; 1966.

Fortun C, Fortun A, Almendros G. The effect of organic materials and their humified fractions on the formation and stabilization of soil aggregates. The Sci. of the Total Envi. 1989;81/82:561-568.

Smidova M. The influence of humus acid on the respiration of plant roots. Biol. Plant. 1960;2:154-164.

Chen Y, Avaid T. Effect of humic substances on plant growth. In: American Society of Agronomy and Soil Science Society of America (Eds.), Humic Substances in Soil and Crop Science; Selected Readings. American Society of Agronomy, Madison, WI. 1990;161-186.

Chen Y, Magen H, Clapp CE. Plant growth stimulation by humic substances and their complexes with iron. Proceedings of International Fertilizer Soci. Israel. 2001;14.

Nardi S, Pizzeghello D, Muscolo A, Vianello A. Physiological effects of humic substances in plant growth. Soil Biol. Biochem. 2002;34(11):1527-1536.

Pettit RE. Organic matter, humus, humate, humic acid, fulvic acid and humin: Their importance in soil fertility and plant health [Online]; 2004. Available:www.humate.info/mainpage.htm

Chen Y, Clapp CE, Magen H, Clinen VW. Stimulation of plant growth by humic substances: Effects on iron availability. In: Ghabbour, EA, Davies G. (Eds.), Understanding humic substances: Advanced methods, properties and applications. Royal Society of Chemistry, Cambridge, UK. 1999;255-263.

Li ZR, Liu YB, Zhou XM, Li XG, Bai LY. Allelopathic herbicidal effects of crude ethanolic extracts of Veronica persica (Lour.) Merr weeds. Allelopath. J. 2019;46:85–96.

Mayhew L. Humic substances in biological agriculture [Online]; 2004. Available:ww.acresusa.com/toolbox/reprints/Jan04_Humic%20Substances.pdf

Kocacaliskan I, Ceylan M, Terzi I. Effects of juglone on seedling growth in intact and coatless seeds of cucumber (Cucumis sativus cv.) Beith Alpha). Academic J. Scientific Res. and Essay. 2009;4(1):39-41.

Alam SM, Ala SA, Azmi AR, Khan MA, Ansari R. Allelopathy and its role in agriculture. J. Biol. Sci. 2001;1(5):308-315.

Rice EL. Allelopathy. Academic Press, New York, USA; 1974.

Olefosdotter M, Navarez D, Moody K. Allelopathy potential in rice (Oryza sativa L.) germplasm. Ann. Appl. Biol. 1995;127(3):546-560.

Zeng RS, Luo SM, Shi YH, Shi MB, Tu CY. Physiological and biochemical mechanism of allelopathy of secalonic acid F. on higher plants. Agron. J. 2001;93:72-79.

Blum U. Fate of phenolic allelochemicals in soils. Chapter 3 p. 57 of action of allelochemicals. Ed. By Francisco, A. Macias, Juan, C.G. Galindo, Jose, M.G. Moilinillo and Horace, Co. Cutler. Published by CRC Press; 2004.

Said-Al Ahl HAH, El Gendy AG, Omer EA. Humic acid and indole acetic acid affect yield and essential oil of dill grown under two different locations in Egypt. J Pharm Sci Res. 2016;8(8):146–157.

Ali IHH. Allelopathic effect of purple nutsedge (Cyperus rotundus L.) weed on some weeds species. Annals of Agri. Sci. Cairo. 2005;50(1):123-134.

Uludag A, Uremis I, Arslan M, Gozcu D. Allelopathy studies in weed science in Turkey - a review. J. Plant Diseases and Protection. 2006;419-426.

Khanh TD, Elzaawely AA, Chung IM, Ahn JK, Tawata S, Xuan TD. Role of allelochemicals for weed management in rice. Allelop. J. 2007;19(1):85-96.

Richards LA. Diagnosis and improvement of saline and alkaline soils. US Salinity Lab. Staff. Agric. Handbook, No. 60 USA; 1954.

Walkley A, Black. An examination of the De Gtjrff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934;37-29.

Jackson ML. Soil chemical analysis. Prentice-Hall India Part. Ltd., New Delhi, India. 1967;205-225.

Jackson ML. Soil chemical analysis. Prentice-Hall Inc., U.S.A.; 1958.

Page AL, Miller RH, Keeny DR. Methods of soil analysis, Part 2 “Chemical and Microbiological Properties,” (2nd Edn), Amer. Soc. Agron. Monograph No. 9, Mascson, Wisconsin USA; 1982.

Olsen SR, Sommers LE. Phosphorus: Phosphorus soluble in sodium bicarbonate. In: Page AL, et al. (Ed.) Methods of Soil Analysis, Part 2. 2nd Ed. Agron. Monogr. G. ASA and SSSA, Madison, WI. 1982;421-422.

Dewis J, Freites F. Physical and chemical methods of soil and water analysis. FAO, Rome, Soil Bulletin. 1970;10.

Deleenher L, De Boodt M. Soil physics. International Training Center for Post Graduate Soil Scientists (ITC-Ghent), Belgium; 1965.

Metzner H, Rau H, Senger H. Untersuchunger Zur Synchronisierbarkeit einzelner pigment- Mangel Mutanten Von Chlorella. Planta. 1965;65:186-194.

Fales FW. The assimilation and degradation of carbohydrates by yeast cells. J, Biol. Chem. 1951;193:113.

Lowry OH, Rosebrough NJ, Farr AL, Ranall RJ. Protein measurement with Metzner, H.; Rau, H. and Senger, H. (1965), Untersuchunger Zur Synchronisierbarkeit einzelner pigment- Mangel Mutanten Von Chlorella. Planta. 1951;65:186-194.

Bates LS, Waldern RP, Teare ID. Rapid determination of free proline for water stress studies. Plant Soil. 1973;39:205-207.

Moore S, Stein W. Photometric ninhydrin method for use in the chromatography of amino acids. J. of Bio. Chem. 187:367-388. The Folin Phenol Reagent. J. Biol. Chem. 1948;193:291-297.

Sndecor GW, Cochran WG. Statistical methods 7th Ed. IOWA, State Univ. U.S.A.; 1990.

Khaled H, Fawy HA. Effect of different levels of humic acids on the nutrient content, plant growth and soil properties under conditions of salinity. Soil and Water Res. 2011;6(1):21–29.

Amlinger F, Peyr S, Geszti J, Dreher P, Karlheinz W, Nortcliff S. Beneficial effects of compost application on fertility and productivity of soils. Literature Study, Federal Ministry for Agriculture and Forestry, Envi. and Water Management, Austria; 2007. [Online] Available: www.umweltnet.at/filemanager/download/20558/ (Dec. 2013)

Brown S, Cottone M. Changes in soil properties and carbon content following compost application: Results of on-farm sampling. Compost Science and Utilization. 2011;19(1):88-97.

Vengadaramana A, Jashothan PTJ. Effects of organic fertilizers on the water holding capacity of soil in different terrains of Jaffna peninsula in Sri Lanka. J. Nat. Prod. Plant Res. 2012;2(4):500-503.

Oo AN, Iwai CB, Saenjan P. Soil properties and maize growth in saline and non-saline soils using cassava-industrial waste compost and vermicompost with or without earthworms. Land Degrad. Dev. 2013;26:300–310. DOI: 10.1002/ldr.2208

Patrick F. Soils, their formation, classification and distribution. Longman, New York. Adv. Soil Sci.; 1983.

Tayel MY, Abdel Hady M. Water movement under saturated and unsaturated flow in coarse textured soils under Baharia Oasis conditions. Egypt. J. Appl. Sci. 2005;20(6A):358-370.

Mohamed WH. Effect of humic acid and calcium forms on dry weight and nutrient uptake of maize plant under saline condition. Austr. J. Basic and Appl. Sci. 2012;6(8):597–604.

Hoda MRMA, Fatma SHI. Effectiveness of humic acid application in improving saline soil properties and fodder beet production. J. Soil Sci. and Agric. Eng., Mansoura Univ. 2016;7(9):623–634.

El-Sherief AA, Tantawy MF, Shaban Kh. A. Improving newly reclaimed sandy saline soil properties and its productivity of Sudan grass by organic, bio and mineral – N fertilization. The Second International Conference On Environmental Studies and Research (Natural Resources & Future Challenges), Environmental Studies and Research Institute (ESRI), University of Sadat City, Egypt, 25–27 February. 2013;249-266.

Abdel-Fattah MK. Role of gypsum and compost in reclaiming saline-sodic soils. J. Agric. Veterinary Sci. 2012;1:30-38.

Brady NC. The nature and properties of soils. (10th Ed.). Macmillan publ. Co., N.Y. 1990;7,8,10,16.

Gulser F, Sonmez F, Boysan S. Effects of calcium nitrate and humic acid on pepper seedling growth under saline condition. J. Envi. Biol. 2010;31:873-876.

Ouni Y, Ghnaya T, Montemurro F, Abdelly Ch, Lakhdar A. The role of humic substances in mitigating the harmful effects of soil salinity and improve plant productivity. Intern. J. Plant Prod. 2013;8(3):353-374.

Amlinger F, Peyr S, Geszti J, Dreher P, Karlheinz W, Nortcliff S. Beneficial effects of compost application on fertility and productivity of soils. Literature Study, Federal Ministry for Agriculture and Forestry, Envi. and Water Management, Austria; 2007. [Online] Available:www.umweltnet.at/filemanager/download/20558/ (Dec. 2013)

Dadhich SK, Somani LL, Shilpkar D. Effect of integrated use of fertilizer P, FYM and biofertilizers on soil properties and productivity of soybean–wheat crop sequence. J. Advances in Develop. Res. 2011;2(1):42-46.

Agegnehu G, Van Beek C, Bird M. Influence of integrated soil fertility management in wheat and productivity and soil chemical properties in the highland tropical environment. J. of Soil Sci. and Plant Nutrition. 2014;14.

Abdel-Rahman G. Impacts of compost on soil properties and crop productivity in the Sahel North Burkina Faso. American-Eurasian J. Agric. and Envi. Sci. 2009;6(2):220-226.

Mohammad HG, Denney MJ, Iyekar C. Use of composted organic wastes as alternative to synthetic fertilizers for enhancing crop productivity and agricultural sustainability on the tropical island of Guam. 13th International Soil Conservation Organization Conference – Brisbane; 2004.

Stevenson FJ. Humus chemistry: Genesis, composition, reaction (Second Ed.), Wiley and Sons, Inc., New York; 1994.

Zaky MH, El-Zeiny OAH, Ahmed ME. Effect of humic acid on growth and productivity of bean plants grown under plastic low tunnels and open field. Egypt. J. Applied Sci. 2006;21(4B).

Boyle M, Frakenburger WT, Stolyz LH. The influence of organic matter on soil aggregation and water infiltration. J. Prod. Agri. 1989;2:290–299.

Schnitzer M. Significance of soil organic matter in soil formation, transport processes in soils and in the formation of soil structure. Soil Utili. and Soil Fertility. Volume 4, Humus Budget. 1992;206:63–81.

Nisar A, Mir S. Lignitic coal utilization in the form of HA as fertilizer and soil conditioner. Sci. Tech. Develop. 1989;8:23–26.

Ibrahim SM, Goh TB. Changes in macro aggregation and associated characteristics in mine tailings amended with humic substances. Common. Soil Sci. Plant Analysis. 2004;35:1905–1922.

Ayuso M, Hernandez T, Garcia C, Pascual J. Stimulation of barley growth and nutrient absorption by humic substances originating from various organic materials. Biores. Technol. 1996;57:251-257.

Sharif M, Khattak RA, Sarir MS. Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Common. Soil Sci. Plant Analysis. 2002;33:3567–3580.

Said-Al Ahl HAA, Hussein MS. Effect of water stress and potassium humate on the productivity or egano plant using saline and fresh water irrigation. Ozean J. Appl. Sci. 2010;3(1):124-141.

William A. The effect of cell cycle regulators on protein profiles in cultured root meristems of Pesium sativum. Environ. Expert. Botany. 1989;29(3):317-322.

Cooper RI, Liu C, Fisher DS. Influence of humic substances on rooting and nutrient content of creeping bent grass. Crop Sci. 1998;38:1639-1644.

Atiye RM, Lee S, Edwards CA, Arancon NQ, Metzger JD. The influence of humic acids derived from earthworm processed organic wastes on plant growth. Soil Ecology Laboratory, 105 Botany and Zoology Building, The Ohio State University, 1735 Neil Avenue, Columbus, OH 43210, USA; 2002.