Chemical Control of Insect Pests by Reduced Doses and Effect on Leaf Components in Alfalfa (Medicago sativa L.)


Published: 2020-02-24

Page: 1-9

Ivelina Nikolova *

Institute of Forage Crops, 89 "General Vladimir Vazov" Str., 5800 Pleven, Bulgaria and Department of Forage Production and Livestock Breeding, Institute of Forage Crops, Pleven, Bulgaria.

Natalia Georgieva

Institute of Forage Crops, 89 "General Vladimir Vazov" Str., 5800 Pleven, Bulgaria and Department of Forage Production and Livestock Breeding, Institute of Forage Crops, Pleven, Bulgaria.

*Author to whom correspondence should be addressed.


Control insect pests in modern agroecosystems are essential for the environment and biodiversity. In this regard, the aim of the study was to determine the effects of Biscaya (thiacloprid 240 g/L), applied alone and with 1/5 and 2/5 reduced doses in a mix with the mineral oil Akarzine on pea aphid, Acyrthosiphon pisum Harr. and alfalfa plant bug, Adelphocoris lineolatus Goeze mortality, plastid pigments and total nitrogen content on alfalfa plants. It was found that mineral oil Akarzine exhibited an insecticidal effect from the first to the seventh day, including after treatment. Akarzine, combined with 1/5 and 2/5 reduced doses of Biscaya, helped to prolong the toxic action of the insecticide and provided a significantly lower density of pea aphid and alfalfa plant bug. Factor days after treatment had a dominant influence and a significant effect on the efficacy - 66.5%. The total pigment content in leaves was statistically reduced after the treatments except for combined Biscaya in the 2/5 reduced dose and Akarzine. The combination retained the green and yellow pigments, did not reduce the photosynthesis efficiency of the plant. The use of Akarzine with Biscaya in 1/5 reduced dose led to the highest nitrogen fixation in alfalfa. The mixture of Acarzine and Biscaya in 1/5 reduced dose was marked by a high protective effect against insect pests, good physiological state of the plants, improved photosynthesis and high nitrogen content.

Keywords: Biscaya, reduced doses, Akarzine, mortality, pigments

How to Cite

Nikolova, I., & Georgieva, N. (2020). Chemical Control of Insect Pests by Reduced Doses and Effect on Leaf Components in Alfalfa (Medicago sativa L.). Asian Journal of Research and Review in Agriculture, 2(1), 1–9. Retrieved from


Download data is not yet available.


Klingler JP, Edwards OR, Singh KB. Independent action and contrasting phenotypes of resistance genes against spotted alfalfa aphid and blue-green aphid in Medicago truncatula. New Phytologist. 2007;173:630–640.

Saini RK, Yadav GS, Kumari B. Novel approaches in pest and pesticide management in agro-ecosystems. CCSHAU Press, Hisar; 2014.

Nikolova I, Georgieva N. Use of preparations with different biological effect in spring vetch and their influence on the productivity and insect pest density. General and Applied Plant Physiology. 2010;36(1-2):30-39.

Demkin AV. Pea aphids and its harmfulness depending on the conditions of mineral nutrition and the use of insecticides. In: Proceedings of the International Conference. Integrated crop protection and pest monitoring in modern agriculture. Stavropol, Agrus. (In Russian). 2007;99–102.

McLaren D. Potato virus Y (PVYO and PVYN:O) impact on potato cultivars and management through oil prays. Agriculture and Agri-Food Canada; 2008.


Tiyagi SA, Ajaz S, Azam MF. Effect of some pesticides on plant growth, root nodulation and chlorophyll content of chickpea. Archives of Agronomy and Soil Science. 2004;50:529-533.

Mishra V, Srivastava G, Prasad SM. Antioxidant response of bitter gourd (Momordica charantia L.) seedlings to interactive effect of dimethoate and UV-B irradiation. Scientia Horticulturae. 2009;120:373–378.

Zelenskii M, Mogileva G. Comparative evaluation of the photosynthetic ability of agricultural crops by the photochemical activity of chloroplasts. VIR, Leningrad (in Russian); 1980.

Gardner-Gee R, Puketapu А, MacDonald F, Connolly P, May P. Effect of selected oils and insecticides on beneficial insect species. A report prepared for: Potatoes New Zealand Ref: SFF11-058: Developing IPM tools for psyllid management in potato. Plant & Food Client Report No: 52837. Plant & Food Research Contract. No. 29597. Job code: P/336016/12. PFR SPTS No. 8405; 2013.

Margaritopoulos JT, Kasprowicz L, Malloch GL, Fenton B. Tracking the global dispersal of a cosmopolitan insect pest, the peach potato aphid. BMC Ecology. 2009;9:13-19.

Suranyi R. Crop borders and mineral oils: Two tactics for management of PVY in seed potatoes; 2010.


Guedes PN, Picanco MC, Guedes NM, Medeira N. Synergism of mineral oil with insecticide toxicity for Scrobipalpuloides absoluta (Lepidoptera: Gelechiidae). Pesquisa Agropecuária Brasileira. 1995;30:313–318.

Pluschkell U, Horowitz AR, Ishaaya I. Effect of milbemectin on the sweet potato whitefly, Bemisia tabaci. Phytoparasitica. 1999;27(3):183-191.

Jeffrey SW, Mantoura RFC, Bjornland T. Phytoplankton pigments in oceanography: Guidelines to modern methods, Jeffrey SW, Mantoura RFC, Wright SW; Eds. UNESCO Publication. 1997; 449-559.

Matile P, Hoertensteiner S, Thomas H. Chlorophyll degradation. Annual Review of Plant Physiology and Plant Molecular Biology. 1999;50:67-95.

Calow P, Sibly R. A physiological basis of population processes: Ecotoxicological implications? Functional Ecology. 1990;4: 283-288.

Mohapatra PK, Patra S, Samantaray PK, Mohanty RC. Effect of the pyrethroid insecticide cypermethrin on photosynthetic pigments of the cyanobacterium Anabaena doliolum Bhar. Polish Journal of Environmental Studies. 2003;12(2):207-212.

Parween T, Jan S, Mahmooduzzafar-Fatma T. Assessing the impact of chlorpyrifos on growth, photosynthetic pigments and yield in Vigna radiata L. at different phenological stages. African Journal of Agricultural Research. 2011;6(19):4432-4440.

Kumar S, Sharma JG. Effect of malathion on seed germination and photosynthetic pigments in wheat (Triticum aestivum L.). Asian Journal of Applied Science and Technology. 2017;1(7): 158-167.

Ilieva A, Vasileva V. Effect of presowing treatment of seeds with insecticides on parameters related to nodulation and nitrate reduction in soybean [Glycine max (L.) Merr.]. Banat's Journal of Biotechnology. 2012;III(6):24-31.

Schulz T, Thelen KD, Difonzo C. Neonicotinoid seed treatments for soybeans. Soybean Seed Potatoes; 2007.


Cen PY, Bornman JF. The response of bean plants to UV-B radiation under different irradiances of background visible light. Journal of Experimental Botany. 1990;411:1489-1495.

Strid A, Porra RJ. Alteration in pigment content in leaves of Pisum sativum after exposure to supplementary UV-B. Plant cell Physiology. 1992;33:1015-1023.

Wardlaw IF. The control of carbon partitioning in plants. New Phytologist. 1990;116:341–381.

Tolley-Henry L, Raper CDJr. Soluble carbohydrate allocation to roots. Photosynthetic rate of leaves, and nitrate assimilation as affected by nitrogen stress and irradiance. Botanical Gazette. 1991;152:23–33.

Graham PH. Ecology of the root-nodule bacteria of legumes. In: Dilworth MJ, James EK, J Sprent WE. Newton (eds). Nitrogen-fixing leguminous symbiosis. Springer, Dordecht, The Netherland. 2008;23-58.

Solomon T, Pant LM, Angaw T. Effects of Inoculation by Bradyrhizobium japonicum strains on nodulation, nitrogen fixation and yield of soybean (Glycine max L. Merill) varieties on nitisols of Bako, Western Ethiopia. International Scholarly Research Network, ISRN Agronomy. 2012;8.

[Article ID: 261475]

DOI: 10.5402/2012/261475