Physicochemical Properties and Microbiological Evaluation of Sausage Prepared from Different Proportion of Chicken and Beef

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Published: 2024-04-04

Page: 79-89

Akinleye, Sule Bamidele *

Department of Animal Science, Meat Science Laboratory, University of Ibadan, Ibadan, Nigeria.

Adeyemi Saheed Abimbade

Department of Animal Science, Faculty of Agriculture, Al-Hikmah University, Ilorin, Kwara State, Nigeria.

Umar Musa Muhammad

Department of Animal Science, Federal University, Kashere, Gombe State, Nigeria.

Usman Grace Ojali

Department of Food, Nutrition and Home Sciences, Kogi State University, Anyigba, Kogi State, Nigeria.

Jibrin Netala

Department of Animal Science, Faculty of Agriculture, Al-Hikmah University, Ilorin, Kwara State, Nigeria.

*Author to whom correspondence should be addressed.


The development of meat industry and meat processing has shifted from marketing of live animals and adopt value added chain in meat products. This study accessed different proportion of beef and chicken breast in sausage production. Twenty kilograms of Pectoralis major and Adductor muscle mixture using five mixing ratio between breast meat and beef. Physicochemical, microbiological and sensory properties were analyzed in this study. The analyses were carried out using SAS software while the means were separated with LSD at 5%. The influence of meat mixture on sausage was significant (P<0.05) among the treatment: proximate composition indicated that treatment I (70 % chicken breast) had the highest percentage of crude protein (31.77±0.65 %) compared to the lowest percentages in treatment V (70% Beef) (27.57±0.55%); pH values were within the range of 6.16± 0.59 (III) to 6.55± 0.21 (V) (P<0.05). Total Aerobic Counts for raw sausages was highest in treatment V (4.63± 0.39 cfu/g) while the lowest value was recorded in I (1.38± 0.08 cfu/g). This study confirmed that pectoralis major can be successfully used in the production of sausage with preference to the consumer acceptability and would also enhance cooking yield and nutritional values.

Keywords: Pectoralis, Adductor, Aerobic, chicken, sausage, microbiological aspects

How to Cite

Bamidele, A. S., Abimbade, A. S., Muhammad, U. M., Ojali , U. G., & Netala , J. (2024). Physicochemical Properties and Microbiological Evaluation of Sausage Prepared from Different Proportion of Chicken and Beef. Asian Journal of Research and Review in Agriculture, 6(1), 79–89. Retrieved from


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Poławska E, Cooper RG, Jóźwik A, Pomianowski J. Meat from alternative Species-Nutritive and dietetic value, and its benefit for human health-A review. CyTA-Journal of Food. 2013;11(1):37–42 Available: 2.680916

Saygın Ö, Demirbaş N. Türkiye de Kırmızı Et Tüketimi: Sorunlar ve Öneriler. Selcuk Journal of Agricultural and Food Sciences. 2018;32(3):567–574. Available: 2018.138

Saygin Ö, Demirbaş N. Türkiye’de Kırmızı Et Sektörünün Mevcut Durumu ve Çözüm Önerileri. Hayvansal Üretim. 2017;58 (1):74–80.

Jung S, Nam KC, Jo C. Detection of malondialdehyde in processed meat products without interference from the ingredients. Food Chem. 2016;209:90–94.

Petracci M. Mudalal S. Bonfiglio A, Cavani C. Occurrence of white striping under commercial conditions andits impact on breast meat quality in broiler Chicken Poultry Science. 2013;92(6):1670-1675

Zorba Ö, Kurt Ş. Optimization of emulsion characteristics of beef, chicken and turkey meat mixtures in model system using mixture design. Meat Sci. 2006;73:611–618.

Büşra Y, Muhittin Z, Makbule G, Özlem VABB, Postacı Ç, Emre O. Chemical composition of meat from different species of animalsnternational Journal of Agriculture, Environment and Food Sciences. 2023;7(3):581-587. Available:

Bhattacharyya D, Sinhamahapatra M, Biswas S. Preparation of sausage from spent duck- an acceptability study. International Journal of Food Science and Technology. 2005;42:24-29.

Ali MS, Kim GD, Seo HW, Jung EY, Kim BW, Yang HS, Joo ST. Possibility of Making Low-fat Sausage from Duck Meat with Addition of Rice Flour. Asian Australian J. of Anim. Sci. 2008;24: 421-428

Ahhmed AM, Kuroda R, Kawahara S, Otha K, Nakade K, Aoki T. Dependence of microbial transglutaminase on meat types in myofibrillar proteins cross- linking. Food Chemistry. 2007;112: 354−361.

Heck RT, Fagundes MB, Cichoski AJ, de Menezes CR, Barin JS, Lorenzo JM, Wagner R, Campagnol PCB. Volatile compounds and sensory profile of burgers with 50% fat replacement by micro particles of chia oil enriched with rosemary. Meat Sci. 2019;148:164–170.

Tarladgis BG, Watts BM, Younathan MS, Dugan LJR. A distillation method for the quantitative determination of malonaldehyde in rancid foods. J. Am. Oil Chem. Soc. 1960;37:44–48.

Rhee KS, Anderson LM, Sams AR. Comparison of flavour changes in cooked and refrigerated beef, pork and chicken meat patties. Meat Sci. 1978;71:392–396.

Murphy EW, Criner PE, Gray BC. Comparisons of methods for calculating retentions of nutrients in cooked foods. J Agric Food Chem. 1975;23(6):1153–1157.

Naveena BM, Muthukumar M, Sen AR, Babji Y, Murthy TRK. Quality characteristics and storage stability of chicken patties formulated with finger millet flour (Eleusine coracana). Journal of Muscle Foods. 2006;17(1):92–104.

Honikel KO. Reference methods for the assessment of physical characteristics of meat. Meat sci. 1998;49:447–457.

Murphy RY, Marks BP, Marcy JA. Apparent specific heat of chicken breast patties and their constituent proteins by differential scanning calorimetry. J. Food Sci. 1998;63:88–91.

Gandi BR. Quality characteristics and microbial status of beef smoked with different plant materials and suya produced from round muscles. A thesis submitted to the school of post graduate studies, ahmadu bello university, zaria, nigeria in partial fulfilment of the requirements for the award of a master’s of science degree in Animal Science. 2014:1-94

A.M.S.A. American Meat Science Association. Research guidelines for cookery, sensory evaluation and instrumental tenderness measurements of fresh beef. Chicago, IL, USA; 1995.

SAS. User’s Guide Statistics. SAS Institute, Inc. Cary, N.C., USA; 2000.

Tornberg E. Effect of heat on meat proteins- implications on structure and quality of meat products: A review. Meat Sci. 2005;70:493–508.

Belichovska D, Belichovska K, Pejkovski Z. Effect of genotype on physico-chemical characteristics of rabbit meat. Meat Technology. 2017;58(1):10–15.

Lima LP, Cruz CL, Farias TJ, Wanderley Júnior MA, Junqueira RS, de Oliveira ARA, Cordeiro CF, Filho JDG. Physicochemical characteristics of lamb meat fed with cottonseed associated with calcium lignosulphonate. Acta Scientiarum-Animal Sciences. 2022;44:1–10. Available:https://doi. org/10.4025/actascianimsci.v44i1.54682

Leygonie C, Britz TJ, Hoffman LC. Meat quality comparison between fresh and frozen/thawed ostrich m. iliofibularis. meat sci. 2012;91:364–368

Parlak Ö, Zorba Ö, Kurt Ş. Modelling with response surface methodology of the effects of egg yolk, egg white, and sodium carbonate on some of the physical-chemical and sensory properties of beef patties. Int. J. Food Eng; 2011. DOI: 10.2202/1556-3758.2004

Yu TY, Morton JD, Clerens S, Dyer JM. Cooking-induced protein modifications in meat. Comprehensive Rev Food Sci. And Food Safety. 2017;16(1):141–159.

Falowo AB. Fayemi PO. Muchenje V. Natural antioxidants against lipid–proteinoxidative deterioration in meat and meat products: A review. Food Res. Int. 2014;64:171–181.

Vargas-Ramella M, Pateiro M, Barba FJ, Franco D, Campagnol PCB, Munekata PES. Tomasevic I, Domínguez R, Lorenz JM. Micro encapsulation of healthier oils to enhance the physicochemical and nutritional properties of deer pâté. LWT. 2020;125

Domínguez R, Pateiro M, Gagaoua M, Barba FJ, Zhang W, Lorenzo JM. Acomprehensive review on lipid oxidation in meat and meat products. Antioxidants. 2019;8:429.

Enser M. What is lipid oxidation. Fd Sci. Technol. Today. 1987;1:151-3.

Nkukwana TT, Muchenje V, Masika PJ, Hoffman LC, Dzama K, Descalzo AM. Fatty acid composition and oxidative stability of breast meat from broiler chickens supplemented with Moringa oleifera leaf meal over a period of refrigeration. Food Chemistry. 2014;142: 255-261.

Kim JH, Yim DG. Assessment of the microbial level for livestock products in retail meat shops implementing HACCP system. Korean Journal for Food Science of Animal Resources. 2016;36: 594-600.

DOI: 10.5851/kosfa.2016.36.5. 594

Rymer C, Givens DI. n-3 fatty acid enrichment of edible tissues of poultry: A review. Lipids, 2005;40(2):121-140. Available:

Peña-Saldarriaga LM, Fernandez Lopez J, Sayas-Barberá E, Pérez-Alvarez JA. Broiler chickens: Characterization of abdominal and gizzard fat. In Proceedings of the 1st World Congress of Food Safety andSecurity, Leiden, The Netherlands. 2019;24–28:76

USDA. Table of Nutrient Retention Factors, Release 6. Prepared by Nutrient DataLaboratory, Beltsville Human Nutrition Research Center (BHNRC), Agriculture Research Service (ARS), U. S. A., Department of Agriculture (USDA); 2007.

Wilkinson BHP, Lee E, Purchas RW, Morel PCH. The retention and recovery of amino acids from pork longissimus muscle following cooking to either 60 °C or 75 °C. Meat Sci 2014;96:361–5.

Smith MO. Parts yield of broilers reared under cycling high temperatures. Poultry Science. 1993;72:1146-1150.

Luchak GL, Miller RK, Belk KE. Hale DS. Michaelswen SA. Johnson DD, West RL, Leak FW, Cross HR, Savell JW. Determination of Sensory, Chemical and Cooking Characteristics of Beef Cuts Differing in Intramuscular and External Fat. Meat Science. 1998;50:55-72

Kempster AJ, Croston D, Jones DW. Tissue growth and development in crossbred lambs sired by ten breeds.Livest Prod Sci. 1987;16:145–162

Harris KB, Harberson TJ, Savell JW, Cross HR, Smith SB. Influences of Quality Grade, External Fat Level, and Degree of Doneness on Beef Steak Fatty Acids. Journal of Food Composition and Analysis. 1991b;5:84-89

Walker SJ, Betts G. Factors affecting the microflora of chilled foods. In: Chilled Foods, Stringer, M. and C. Dennis (Eds.) wood head publishing. London. 2000;157-178

Syne SM, Ramsubhag AA, Adesiyun AA. Microbiological hazard analysis of ready-to-eat meats processed at a food plant in Trinidad, West Indies. Infection Ecology and Epidemiology. 2013;3:20450 Available:

NTC. 1325 Norma Técnica Colombiana Para La Industria Alimentaria: Productos Cárnicos Procesados no Enlatados; ICONTEC: Bogota, Colombia; 2008.

A.O.A.C. Official Methods of Analysis. Association of Official Analytical Chemists, 17th edition. Washington, DC, USA; 2000

APHA. American Public Health Association Compendium of Methods for the Microbiological Examination of Foods, 4th edition. Washington D. C., USA. 2002;7-97:239-250 and 325-422.

Bendall JR. In: Bourne GH. (Ed). Structure and function of muscle, 466 Academic Press, New York, 2nd Edition. 1973;2(2):243-309.

Federal Register. Substances for use in meat and poultry products. 1979;44 (151,Ang.3), 45606-7.

ICMSF. Sampling plans for fish and shellfish. In: Microorganisms in Foods. Sampling for Microbiological Analysis: Principles and Scientific Applications (edited by ICMSF), 2nd Edition. Toronto, Canada: University of Toronto Press; 1986:181–196.

Jung E, Joo N. Roselle (Hibiscus sabdariffa L.) and soybean oil effects on quality characteristics of pork patties studied by response surface methodology. Meat Science. 2013;94:391-401.