Haematological Alterations in Common Carp (Cyprinus carpio) Infected by Saprolegnia spp.


Published: 2023-12-31

Page: 99-105

Muhammad Zubair Anjum *

Department of Zoology and Biology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.

Muhammad Junaid

Department of Zoology and Biology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.

Usman Akhtar Butt

Department of Zoology and Biology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.

Muhammad Irfan

Department of Zoology and Biology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.

Zahir Muhammad

Department of Zoology and Biology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.

Muhammad Qayash Khan

Department of Zoology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan, Pakistan.

*Author to whom correspondence should be addressed.


The effect of Saprolegniasis on hematological parameters of Cyprinus carpio was studied in River Indus at Swabi, Khyber Pakhtunkhwa, Pakistan. The results showed that Saprolegniasis significantly decreased the total erythrocytes count, packed cell volume, and hemoglobin content, while the white blood cells and mean corpuscular volume were significantly increased in the infected fish as compared to the healthy fish. Mean corpuscular hemoglobin was found higher while mean corpuscular hemoglobin concentration was found lower in infected fish. Saprolegnia triggers a strong inflammatory response in its host by suppressing fish immunity. The Pearson linear correlation analysis showed a significant correlation for several parameters (P<0.05). It is obvious that Saprolegniasis seriously damage the population of freshwater fishes.

Keywords: Cyprinuscarpio, saprolegniasis, hematology, histopathology

How to Cite

Anjum, M. Z., Junaid, M., Butt, U. A., Irfan, M., Muhammad, Z., & Khan, M. Q. (2023). Haematological Alterations in Common Carp (Cyprinus carpio) Infected by Saprolegnia spp. Asian Journal of Research and Review in Agriculture, 5(1), 99–105. Retrieved from https://globalpresshub.com/index.php/AJRRA/article/view/1961


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Fisheries, FAO. The state of world fisheries and aquaculture. Rome: Food and Agriculture Organization of the United Nations; 2018.

Kwasek K, Thorne-Lyman AL, Phillips M. Can human nutrition be improved through better fish feeding practices? A review paper. Critical Reviews in Food Science and Nutrition. 2020;60(22):3822-3835. DOI: 10.1080/10408398.2019.1708698

Basumatary M, M. Gogoi S. Chakravarty. In vitro study on dermal lesion disease of Puntius spp. of Tarang River: A tributary of Champabati. IJAR. 2015;1(7):65-68.

Boyle H. WWF: World’s Top 10 Rivers at Risk; 2007. Available:http://wwf.panda.org/about_our_earth/about_freshwater/freshwater_problems/river_decline/10_rivers_risk/indus/

Bruno DW, Wood BP. Saprolegnia and other Oomycetes. In: Woo PTK, Bruno DW, editors. Fish diseases and disorders, Viral, Bacterial and Fungal Infections. Owon, United Kingdom: CABI Publishing, Wallingford. 1999;3:599-659.

Hussein MMA, Hatai K, Nomura T..Saprolegniois in salmonids and their eggs in Japan.Journal of Wildlife Diseases. 2001;37(1):204-207. DOI:10.7589/0090-3558-37.1.204

Khan A, Yousafzai AM, Latif M, Rehman AU, Khan Q, Zaib A, Ullah A, Sthanadar AA, Haq IU, A. Aziz. Analysis of selected water quality parameters and heavy metals of Indus River at Beka Swabi, Khyber Pakhtunkhwa, Pakistan. International Journal of Biosciences. 2014;4(2):28-38. DOI: 10.12692/ijb/4.2.28-38

Ganguly S.R. Wakchaure PK. Praveen, PA. Para. Fungal infections in fishes: A Brief Review. International Journal of Pharmacy & Life Sciences. 2016;7(9).

Willoughby LG. Saprolegnias of salmonid fish in Windermere: A critical analysis. Journal of Fish Diseases. 1978;1(1):51-67. DOI: 10.1111/j.1365-2761.1978.tb00005.x

Yeasmin SM, Md. A. Rahman, Md MM. Hossain Md. H. Rahman AA. Asif. Identification of causative agent for fungal infection and effect of disinfectants on hatching and survival rate of common carp (C. carpio) larvae. Asian J. Med. Biol. Res. 2015;1(3):578-588. DOI: 10.3329/ajmbr.v1i3.26481

Callinan RB Sammut J, Fraser GC. Dermatitis, branchitis and mortality in empire gudgeon Hypseleotriscompressa exposed naturally to runoff from acid sulfate soils. Diseases of aquatic organisms. 2005;63(2-3):247-253.

Nwabueze AA, Olele NF, Ekelemu JK. Saprolegniasis in freshwater Catfishes sold in fish markets in Asaba, Southern Nigeria. I.J.A.B.R. 2013;3(2):204-207. DOI:10.12735/AS.V1I2P10.

Magray AR, Hafeez S, Ganai BA, Lone SA, Dar GJ, Ahmad F, Siriyappagouder P. Study on pathogenicity and characterization of disease causing fungal community associated with cultured fish of Kashmir valley, India. Microbial Pathogenesis. 2021;151:104715. DOI: 10.1016/j.micpath.2020.104715

Kalatehjari P, Yousefian M, Khalilzadeh MA. Assessment of antifungal effects of copper nanoparticles on the growth of the fungus Saprolegnia sp. on white fish (Rutilusfrisiikutum) eggs. Egyptian Journal of Aquatic Research. 2015;41: 303–306. DOI: 10.1016/j.ejar.2015.07.004

Sharma M, Shrivastav AB, Sahni YP, Pandey G. Overviews of the treatment and control of common fish diseases. International Research Journal of Pharmacy. 2012;3(7):123-127.

Moghaddam AA, Hajimoradloo A, Ghiasi M, Ghorbani R. In vitro Inhibition of Growth in Saprolegnia sp. Isolated from the Eggs of Persian Sturgeon Acipenserpersicus (Pisces: Acipenseriformes) by Pseudomonas aeroginosa (PTCC: 1430). Caspian J. Env. Sci. 2012;11(2):233-240. DOI: 10.13140/RG.2.2.25018.31682

Jamalzadeh HR, Keyvan A, Ghomi MR, Gherardi F. Comparison of blood indices in healthy and fungal infected Caspian salmon (Salmotrutta caspius). African Journal of Biotechnology. 2009;8(2).

Chauhan R, Faroq A, Lone SA, Ganaie SA. Hematological and Histological investigations on healthy and Saprolegnia sp. infected Clarias gariepinus (BURCHELL1822). 2014;4(2);97-100.

Ali SE, Evensen Ø, Skaar I. Recent advances in the mitigation of Saprolegnia infections in freshwater fish and their eggs. Formatex. 2015;691-697.

Singh R, Pandey NN, Gupta M. Hematological changes in common carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss), Infected with Saprolegnia spp; 2013. DOI: 10.46989/001c.20670

Shah SL, Altindag A. Haematological parameters of tench, (Tincatinca L.) after acute and chronic exposure to lethal andsublethal mercury treatments. Bull. Environ. Contam. Toxicol. 2004;73:(1) 911-918. DOI: 10.1007/s00128-004-0513-y

McLeay DJ. Effects of a 12-hr and 25-day exposure to kraft pulp mill effluent on the blood and tissues of juvenile coho salmon (Oncorhynchus kisutch). Journal of the Fisheries Board of Canada. 1973;30(3):395-400.

Gill TS, Epple A. Stress-related changes in the haematologicalprofile of the American eel (Anguilla rostrata). Ecotoxicol.Environ. Saf. 1993;25:227-235. DOI:10.1006/eesa.1993.1021

Palikova M, Navratil S. Occurrence of Anguillicolacrassus in the water reservoir Korycany (Czech Republic) and its influence on the health condition andhematological indices of eels. Acta Vet.Brno. 2001;70:443-449. DOI: 10.1002/aheh.19890170116.

Chen X, Yin D, Hu S, Hou Y.. Immunotoxicity of pentachlorophenol on macrophage immunity and IgM secretion of the crucian carp (Carassius auratus). Bull. Environ. Contam.Toxicol. 2004;73: 153-160. DOI: 10.1007/s00128-004-0407-z

Hatai K, Nakamura K, Rha SA, Yuasa K, Wada S. Aphanomyces infection in dwarf gourami (Colisalalia) Division of Fish Diseases, Nion Veterinary and Animal SciencUniversity,1-7-1 Kyonan-Cho, Musashino,Tokyo 180, Japan; 1994. DOI:10.3147/jsfp.29.95

Hussian, Hassan MMA, WH, Mahmood MA. Pathogenicity of Achlyaproliferoids and Saprolegnia diclina (Saprolegniaceae) associated with saprolegniasis outbreaks in cultured Nile Tilapia (Oreochromis niloticus). World J of Fish and Marine Science. 2013;5(2):188-193. DOI: 10.5829/idosi.wjfms. 2013.05.02.7212

Martins ML, DH Xu, CA. Shoemaker, Klesius PH. Temperature effects on immune response and hematological parameters of channel catfish Ictaluruspunctatus vaccinated with live theronts of Ichthyophthiriusmultifiliis. Fish & shellfish immunology. 2011;31(6):774-780.

Reddy PM, Bashamohideen M. Fenvalerate and cypermethr ininduced changes in the hematological parameters of Cyprinuscarpio. Act. Hydrochim. Hydrobiol. 1989;17:101-107.

Schalm O..Schalm's Veterinary Hematology, 4th Edition. 1986;524. DOI: 10.12691/ajmsm-7-2-2

Shah SL. Impairment in the haematological parameters of tench (Tincatinca) infected by Saprolegnia spp. Turk. J. Vet. Anim. Sci. 2010;34(4):313-318. DOI:10.3906/vet-0706-4

Tacon AG, Metian M. Fish matters: Importance of aquatic foods in human nutrition and global food supply. Reviews in Fisheries Science. 2013;21(1): 22-38. DOI: 10.1080/10641262.2012.753405