Antioxidant Potential of Spathodea campanulata Plant Parts and its Hepatotoxicity on Induced Albino Rats

PDF

Published: 2023-12-31

Page: 86-92


Isaac John Umaru *

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Helen Adeseye Oluwabumi

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Yakubu, Ojochenemi Ejah

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Kerenhappuch Isaac Umaru

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Pamela Ephraim Nyifi

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Stephen Bitrus Hundi

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Monica Okono Etegin

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

Rimamskep Danbeki

Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University, Wukari, Taraba State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Medicinal plants constitute an effective source of both traditional and modern medicine in the present study The African tulip tree, Spathodea campanulata was evaluated for antioxidant potential and its hepatotoxicity on induced albino rats. The flower, leaves, stem bark and roots of Spathodea campanulata were collected, washed, air-dried and pulverized to powder and each extracted with ethanol. Thirty male albino rats weighing (150-190g) were used to carry out this work. The rats were distributed into five (5) groups containing 5 rats per each group. Group I serve as the normal group. Group 2 serve as the negative control and were induced with ulcer without treatment. Group 3 served as the positive control and were induced with ulcer and treated with the reference drug (Omeprazole). Group 4 and 5 were induced with ulcer and treated with 200mg and 400mg of the extracts of the flowers, leaves, stem bark and roots of Spathodea campanulata. The various treatment was administered orally and lasted for a period of two weeks. After the treatment the rats were anaesthetized, sacrificed and blood samples was collected through cardiac puncture and dispersed into an anticoagulant tube and the centrifuged for 10min at 400rpm to obtain a clear serum for biochemical analysis of the indices of the liver function. The antioxidant activity was performed by DPPH (1, 1 diphenyl-2-picryl hydrazyl) radical scavenging method for the different plant parts of Spathodea campanulata. All extracts showed a significant DPPH scavenging activity with increasing concentration. The flower extract showed the highest DPPH scavenging activity (77.57 % RSA) at a concentration of 100μg/ml. The comparative effect of the flower and root extract showed elevated levels of Aspartate amino transferase (AST), Alanine amino transferase (ALT) and Alkaline phosphatase (ALP) during flower extract administration and normal levels of Aspartate amino transferase (AST) and Alanine amino transferase (ALT) during root extract administration indicating it as good natural source for protection of the functioning of the liver.

Keywords: Antioxidant, Spathodea campanulata, hepatotoxicity, albino rats


How to Cite

Umaru, I. J., Oluwabumi , H. A., Ejah, Y. O., Umaru , K. I., Nyifi , P. E., Hundi , S. B., Etegin , M. O., & Danbeki , R. (2023). Antioxidant Potential of Spathodea campanulata Plant Parts and its Hepatotoxicity on Induced Albino Rats. Asian Journal of Research in Medicine and Medical Science, 5(1), 86–92. Retrieved from https://globalpresshub.com/index.php/AJRMMS/article/view/1981

Downloads

Download data is not yet available.

References

Angela E. Peter, Jgan Mohan, Swarajya Lakshmi G, Ganga Roa B, Sandeep V. Preliminary phytochemical, Antioxidant and Antibacterial studies on the bark of Spathodea campanulata P. Beauv. European Journal of Biomedical and Pharmaceutical Sciences. 2016;3(2):243-251

Akroum S, Salta D, Lalaoui K. Antimicrobial, antioxidant, cytotoxic activities and phytochemical screening of some Algerian plants. European Journal of scientific Research. 2007;31: 289-295.

Souri E, Amin G, Farsam H, Barazandeh T. Screening of Antioxidant activity and Phenolic contents of 24 medicinal plant extracts. Daru. 2008;16: 83-87.

Ndhlala A, Ncube B, Vanstaden J. Antioxidants versus reactive oxygen species. A tug of war for human benefits? In: Laher I Editors. System biology of free radicals and antioxidants. Berlin Heidelberg; Springer. 2014;3987-4002.

Patience O, Festus B, Philip F, Nneka R, Ijeoma E, Nkemakonam C. Phytochemical analysis, Hepatoprotective and antioxidant activity of Alchornea cordifolia Methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pacific Journal of Tropical Medicine. 2012; 5:289-293.

Ibrahim M, Khaja M, Aara A. Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: in vitro and In vivo studies. World Journal of Gastroenterology. 2008;14: 2566–2567.

Lawal B, Shittu O, Oibiokpa I, Mohammed H, Umar S, Haruna G. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum. Journal of Acute Disease. 2016;05:002.

Rajkapoor B, Venugopal J, Anbu N, Harikrishman M, Ravichandran V. Protective effect of phyllanthus polyphllus on acetaminphen induced hepatotoxicity in rats. Pakistan Journal of Pharmaceutical Sciences. 2008;21:90.

Bhajoni PS, Lahkar M, Mashram GG. Evaluation of the Antiulcer Activity of the leaves of Azadirachta indica: An Experimental Study. Integrative Medicine International. 2016;3(2):10-16.

Umaru IJ, Fasihuddin AB, Zaini BA. Effect of Leptadenia hastata hexane leaf extracts against haematological, biochemical and indomethacin induced ulcer in rats. European Journal of Pharmaceutical and Medical Research. 2018;5(5):35-41.

Sangeetha S, Meenakshi S, Akshaya S, Vadivel V, Brindha P. Evaluation of total phenolic content and antioxidant activity of different solvent extracts of leaf material of Spathodea campanulata P. Beauv. and investigation of their proliferation inhibition potential against EAC cell line. Journal of Applied Pharmaceutical Science. 2016; 6:121-127.

Bandyopadhyay SK, Pakrashi A, Satyesh C. The role of antioxidant activity of Phyllanthus emblica fruits on prevention from Indomethacin induced gastric ulcer. Journal of Ethnopharmacology. 2020; 70(2):171-176.

Berinyuy EB, Lawal B, Mohammed H, Oibiokpa FI, Shittu OK. African natural products with potential antioxidants and hepatoprotectives properties: A review. Clinical Phytoscience. 2017;2: 1-66.

Faich G, Goldstein JL, Silverstein FE, et al. Gastrointestinal toxicity with celecoxib vs nonsteroidal anti-inflammatory drugs for osteoarthritis and rheumatoid arthritis: the CLASS study: A randomized controlled trial. Celecoxib Long-term Arthritis Safety Study. JAMA. 2020;284: 1247-55.