Positive RT-PCR for COVID-19 in the Exhumed Body: A Dilemma in Dead Body Safety, Virus Survival, and Genome Stability

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Published: 2021-12-11

Page: 71-77


Manjula S. *

Department of Oral and Maxillo-Facial Surgery, JSS Dental College and Hospital, JSSAHER, Mysore, Karnataka, India.

Somanna Ajjamada

Department of Genetics and Genomics, Manasa Gangothri, University of Mysore, Mysore, Karnataka, India.

T. S. Kiran

Department of Microbiology, Sridevi Institute of Medical Sciences and Research Hospital, Tumkuru, Karnataka, India.

D. R. Mahadeshwara Prasad

Department of Forensic Medicine & Toxicology, District Hospital and Designated COVID-19 Hospital, Mysore, Karnataka, India.

*Author to whom correspondence should be addressed.


Abstract

Background: Most cases of COVID-19 could test positive for RT-PCR, weeks later, a possibility of an overestimation during the pandemic. RT-PCR results per se make it difficult to take proper decisions in the admission, treatment, and isolation of the cases. The constantly emerging new data about COVID-19 will dictate the strategies on quarantine, home isolation, hospitalization, and treatment protocol. The present study describes the finding that RT-PCR was positive for the COVID-19 virus genome among the swabs and the visceral samples processed after a total of 38 days.

Methods: A medico-legal exhumation was conducted, and the samples were analyzed for the coronavirus genome through RT-PCR.

Results: The RT-PCR test was positive for the presence of COVID-19 genome with a Cycle threshold (Ct) value of ≤ 35 which was used as a reference to declaring the test positive.

Discussion: The RNA viruses with a complex genomic structure have a wide host range and are exposed to various immune and ecological systems. Multiplicity Reactivation (MR) of the virus is not uncommon. It is quite difficult to infer the safety and herd immunity in the case of COVID-19 due to the concept of MR, a genome repair process. However, in the present study the RT-PCR test being positive needs explanation and draws the attention of the researchers concerning re-infection, genomic stability, immunogenetics, false positives, isolation and management, safe disposal of the deceased body, and bio-medical waste.

Keywords: Dead body safety, genome stability, COVID-19, positive RT-PCR, Multiplicity Reactivation


How to Cite

S., M., Ajjamada, S., Kiran, T. S., & Prasad, D. R. M. (2021). Positive RT-PCR for COVID-19 in the Exhumed Body: A Dilemma in Dead Body Safety, Virus Survival, and Genome Stability. Asian Journal of Research in Medicine and Medical Science, 3(1), 71–77. Retrieved from https://globalpresshub.com/index.php/AJRMMS/article/view/1368

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References

Ministry of Health and Family Welfare. RT-PCR test detects virus even after it becomes inactive and non-infectious: ICMR, explaining new discharge policy. Available:https://pib.gov.in/PressReleasePage.aspx?PRID=1625264; cited on Dec 19th, 2020.

Infectious Disease Physicians (May 23rd, 2020) Academy of Medicine, and the National Center for Infectious Diseases (NCID), Singapore

covid-19-triggered-mucormycosis-cases-rise-in-India-symptoms-treatment-and-risk-factors-of-deadly-black-fungal-infection. Available:https://www.firstpost.com/health/-9123951.html

Ge H, Wang X, Yuan X, Xiao G, Wang C, Deng T. The epidemiology and clinical information about COVID-19. Eur J Clin Microbiol Infect Dis. 2020;39(6):1011-1019.

Hassan SA, Sheikh FN, Jamal S, Ezeh JK, Akhtar A. Coronavirus (COVID-19): A review of clinical features, diagnosis, and treatment. Cureus. 2020;12(3):e7355.

Manjula S, Mahadeshwara Prasad DR, Chandan SN, Shetty SK, ShyamSunder, Shivananda S. Ethical Issues In Covid-19: Implications for the disease control. International Journal of Scientific Research. July 2020;9(7).

Alenquer M, Vale-Costa S, Etibor TA, Ferreira F, Sousa AL, Amorim MJ. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun. Apr 2019;9:10(1):1629.

Bolte H, Rosu ME, Hagelauer E, García-Sastre A, Schwemmle M. Packaging of the Influenza Virus Genome Is Governed by a Plastic Network of RNA- and Nucleoprotein-Mediated Interactions. J Virol. Feb 2019;5:93(4):e01861-18.

Silvia VC, Marta A, Ana LS, Bárbara K, Jose R, Erin MT, Maria JA. Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors. Published by The Company of Biologists Ltd. Journal of Cell Science Advance; 2016.

Brian DA, Baric RS. Coronavirus genome structure and replication. Curr.Topics Microbiol. Immunol. 2005;287:1–30.

Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N, Bi Y, Ma X, Zhan F, Wang L, Hu T, Zhou H, Hu Z, Zhou W, Zhao L, Chen J, Meng Y, Wang J, Lin Y, Yuan J, Xie Z, Ma J, Liu WJ, Wang D, Xu W, Holmes EC, Gao GF, Wu G, Chen W, Shi W, Tan W.. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. Feb 2020;22:395(10224):565-574.

Naqvi AAT, Fatima K, Mohammad T. Insights into SARS-CoV-2 genome, structure, evolution, pathogenesis and therapies: Structural genomics approach. Biochim Biophys Acta Mol Basis Dis. 2020;1866(10):165878.

McClain ME, Spendlove RS. Multiplicity reactivation of reovirus particles after exposure to ultraviolet light. J. Bacteriol. Nov 1966;92(5):1422–9.

Michod RE, Bernstein H, Nedelcu AM. Adaptive value of sex in microbial pathogens. Infection, Genetics and Evolution. 2008;8(3):267–285.

Elsoukkary SS, Mostyka M, Dillard A, Berman DR, Ma LX, Chadburn A, Yantiss RK, Jessurun J, Seshan SV, Borczuk AC, Salvatore SP. Autopsy findings in 32 patients with COVID-19: A single-institution experience. Pathobiology. 2021;88:56–68.

Lindner D, Fitzek A, Bräuninger H. Association of cardiac infection with sars-cov-2 in confirmed COVID-19 autopsy cases. JAMA Cardiology. Nov 2020; 5(11):1281-1285

Casagrande M, Fitzek A, Püschel K, Aleshcheva G, Schultheiss HP, Berneking L, Spitzer MS, Schultheiss M. Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients. Ocul Immunol Inflamm. July 2020;3;28(5):721-725

covid-19-triggered-mucormycosis-cases-rise-in-india-symptoms-treatment-and-risk-factors-of-deadly-black-fungal-infection. Available:https://www.firstpost.com/health/-9123951.html