Genetic Analysis of Eight Parents and their 28 Hybrids under Salinity Tolerance in Bread Wheat

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El Ameen M. Tharwat
Abdelsabour G. A. Khaled
Ibrahim F. O. Elshazly


This investigation was carried out to study the gene action under salinity soil and water on plant height, number of tillers/plants, stem diameter, 100-grain weight, and grain yield/plant, at experimental farm faculty of Agriculture, south valley university, Qena, Egypt during the two winter seasons (2018-2019). The results showed that the mean squares of 36 genotypes were highly significant for all studied traits, reflecting a great wide genetic variability among them. The mean sum of squares due to the general combining ability (GCA) and specific combining ability (SCA) were highly significant for all studied traits. The additive genetic variance (σ2A), which were higher than those of non-additive (σ2D) ones for plant height, stem diameter and 100-grain weight. The values of heritability in broad senses (h2b. s %) and narrow senses (h2n. s%) were estimated for all studied. The results exhibited that genotypic correlation for plant height was highly significant with number of tillers/plants, stem diameter, and grain yield/plant with the values of (0.40, 0.33 and 0.33) respectively, under salinity condition. However, genotypic correlation for stem diameter was positive and highly significant with 100-grain weight with the value of (0.48) under salinity condition. Additionally, the genotypic and phenotypic correlations were positive and highly significant between number of tillers/plants with grain yield/plant with the values of (0.37) and (0.43). Finally, it could be concluded that the additive gene action played a major role in controlling of most traits studied.

Combining ability, heritability, salinity tolerance, stem diameter, wheat

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How to Cite
Tharwat, E. A. M., Khaled, A. G. A., & Elshazly, I. F. O. (2020). Genetic Analysis of Eight Parents and their 28 Hybrids under Salinity Tolerance in Bread Wheat. Asian Journal of Research in Biosciences, 2(1), 37-48. Retrieved from
Original Research Article


Breiman A, Graur B. Wheat evolution. Israel Journal of Plant Science. 1995; 43:85- 98.

Ghassemi F, Jakeman AJ, Nix HA. Salinization of land and water resources: human causes, management and case studies. CABI/ Univ, New South Wales; 1995.

Arzani A. Improving salinity tolerance in crop plants: A biotechnological view. In vitro Cell Dev. Biol. Plant. 2008;44:373-383.

Munns R. Genes and salt tolerance: bringing them together. New Phytologist. 2005;167:645-663.

Inamullah A, Mohammad H, Siraj-ud-Din F Hassan G, Gul R. Evaluation of the heterotic and heterobeltiotic potential of wheat genotypes for improved yield. Pak. J. Bot. 2006;38:1159-1167.

Fehr WR. Principles of Cultivar Development. 1. MacMillan Publ. Co. New York, USA; 1993.

Ashraf M. Genetic variation for salinity tolerance in spring wheat. Hereditas. 1994;120:99-104.

Jinks JL. The analysis of continuous variation in diallel cross in Nicotiana rustica varieties. Genetics. 1954;39:767-788.

El-Sherbeny AR Galal. The relative importance of additive and non- additive genetic variances for breeding new variaties of wheat (Triticum Spp. L.). Thesis Ph.D. in Department of Genetics, Fac, Agric, Mansoura Univ; 1996.

Abd El- Mohsen Ashraf A, Abo Hegazy Samir R, Taha Moemen H. Genotypic and phenotypic interrelationships among yield and yield components in Egyptian bread wheat genotypes. Journal of Plant Breeding and Crop Science. 2012;4(1):9-16.

Steel RG, Torrie JH. Principal and procedures of statistics. Mc Grow Hill Book Inc., New York, USA; 1960.

Cochran WG, Cox GM. Experimental Designs. Wiley, New York; 1957.

Griffing B. Concept of general and specific combining ability in relation to diallel crossing systems. Austrlian Journal of Biological Sciences. 1956;9:463-493.

Matzinger D, Kempthorne O. The modified diallel table with partial inbreeding and interaction with environment. Genetics. 1956;41:822-833.

Singh D. Diallel analysis for combining ability over environments. Indian Journal of Genetics and Plant Breeding. 1979;39:383-386.

Fischer RA, Maurer R. Drought resistance in spring wheat cultivars. I-Grain yield responses. Australian J. of Agricultural Research. 1978;29:897-912.

Shannon MC. Breeding, selection and the genetics of salt tolerance. In: Staple RC, Toeniessen GA (eds), Salt tolerance in plant - Strategis for crop Improvement. Wiley, New York, USA. 2001;231-254.

Sharma AK, Garg DK. Genetic variability in wheat (Triticum aestivum L.) crosses under different normal and saline environment. Annals Agric Res. 2002;23:497-499.

Ashraf M, Harris PJC. Abiotic stresses plant resistance through breeding and molecular approaches. Food Products Press, an imprint of The Haworth Press, Inc. Binghamton, New York, USA. 2005;3–15.

Ahmed MSH. and Mohamed SMS. Genetic analysis of yield and its components diallel crosses of bread wheat under two sowing dates. Egypt. J. Plant Breed. 2009;13:281–301.

El-Hendawy E Salah, Yuncai Hu, Yakout Gamal M, Awad Ahmed M, Hafiz Salah E, Schmidhalter Urs. Evaluating salt tolerance of wheat genotypes using multiple parameters. Europ. J. Agronomy. 2005;22:243–253.

Hamam KA, Negim O. Evaluation of wheat genotypes under saline water irrigation. Annals of agriculture science. 2014;59(2):165-176.

Munir Ahmad, Muhammad Iqbal, Armghan Shahzad, Muhammad Asif and Muhammad Sajad. Genetic analysis of yield and yield contributing quantitative traits in bread wheat under sodium chloride salinity. Journal of Agricultural Science. 2013;5(6).

Elshafei Adel Ahmed, Samy Abd El-Aziz Afiah, Abdullah Abdulaziz Al-Doss, Eid Ibrahim Ibrahim. Morphological variability and genetic diversity of wheat genotypes grown on saline soil and identification of new promising molecular markers associated with salinity tolerance. Journal of Plant Interactions. 2019;14(1):564– 571.

El-Sherbeny AR Galal, Khaled GAA, Haitham MA Elsayed. Inheritance of gene action for yield component traits in bread wheat under normal and drought stress conditions. International Journal of Modern Agriculture. 2019;8(2):2305-7246.

Ljubicic, N, Petrovic S, Kostic M, Dimitrijevic M, Hristov N, Kondic-Spika A, Jevtic R. Diallel analysis of some important grain yield traits in bread wheat crossed. Turkish Journal of Field crops. 2017;22(1): 1-7.

Fellahi Z, Hannachi A, Bouzerzour H, Dreisigacker S, Yahyaoui and Sehgal D. Genetic analysis of morpho-physiological traits and yield components in F2 partial diallel crosses of bread wheat (Triticum aestivum L.). Rev. Fac. Nac. Agron. 2017; 70(3):8237-8250.

Gomaa MA, El-Banna MNM, Gadalla AM, Kandil EE, Ibrahim ARH. Heterosis, combining ability and drought susceptibility index in some crosses of bread wheat (Triticum aestivum L.) under water stress conditions. Middle East Journal of Agriculture Research. 2014;3(2):338-345.

Singh Sanjay, Sengar RS, Neeraj Kulshreshtha, Datta D, Tomar RS, Rao-Deepa Garg VP, Ashish Ojha. Assessment of multiple tolerance indices for salinity stress in bread wheat (Triticum aestivum L.). Journal of Agricultural Science. 2015; 7(3).

Abu-El-lail FFB, Hamam KA, Kheiralla KA, El-Hifny MZ. Salinity tolerance in 280 genotypes of two-rows Barley. Egyptian Journal of Plant Breeding. 2014;18(2):331-345.

Gadallah A Maha, Sanaa I Milad, Mabrook YM, Abo Yossef Amira Y, Gouda MA. Evaluation of Some Egyptian Bread Wheat (Triticum aestivum L.) Cultivars under Salinity Stress. Alexandria Science Exchange Journal. 2017;38(2).

El-Sherbeny AR Galal, Khaled GAA, Haitham MA Elsayed. Identifiction of heterosis and nature of gene action in bread wheat under normal and drought stress. International Journal of Agriculture & Agribusiness. 2019;3(1):2391-3991.

Afiah SAN, Darwish IHI. Combining ability analysis and heterosis in relation to salinity and drought stress for yield and its attributes of breed wheat. J. Agric. Sci. Mansoura, Univ. 2002;27:8033-8049.

Hamam KA, Khaled Abdel-Sabour GA, Ibrahim MS. Combining ability and drought tolerance in bread wheat under water regimes. 6th International Plant Breeding Conference, Ismalia, Egypt; 2009.

Kulshreshtha N, Singh KN. Combining ability studies in wheat (Triticum aestivum L.) for genetic improvement under salt stress. J. Wheat Res. 2011;3(2):22.

Jaiswal KK, Markeri S, Kumar B. Combining ability in diallel crosses of wheat (T. aestivum L.). Supplement Genet. And Pl. Breeding. 2013;8:1557-1560.

Samir KA, Ismail. Heterosis and combining ability analysis for yield and it components in bread wheat (Triticum aestivum L.). Int. J. Curr. Microbio. App. Sci. 2015;4(8):1- 9.

Kandil AA, Sharief AE, Hasnaa SM. Estimation of general and specific combining ability in bread wheat (T. aestivum L.). Int. J. Agri. R. 2016;37.

Okasha SA and Hussein MA. Combining ability and heterosis estimates of some wheat genotypes under salinity stress conditions. Egypt. J. Plant Breed. 2017; 21(4):769– 795.

Adel MM, Ali EA. Gene action and combining ability in a six parent diallel cross of wheat. Asian J. of Crop Sci. 2013; 5:14-23.

Afiah SAN, Mohamed NA, Salem MM. Statistical genetic parameters, heritability and graphical analysis in 8x8 wheat diallel crosses under saline condition. Ann. Agric. Sci Cairo. 2000;45:257-280.

Hassani M, Saeidi G, Rezai A. Estimation of genetic parameters and combining ability for yield and yield components in bread wheat. Journal of Science & Technology Agriculture & Natural Resources. 2005;9:157-171.

Marzooghian Akbar, Mohammad Moghaddam, Mahmoud Toorchi, Mohammad Reza Shakiba. Investigation of genetic structure and gene action in bread wheat affected by salt stress. International Journal of Biosciences. 2014; 5(6):173-181.

Saied Abd El-Rahman Shrief, Mohamed Abd El-Maboud Abd El-Shafi, Sawsan Abd El-Bady El-Sadi Heterosis, gene action and combining ability of grain yield and its components in six bread wheat crosses. Bioscience Research. 2017;14(4):1204-1215

Korkut KZ Varieties, Başer I, Bilgin O. Genotypic and phenotypic variability, heritability and phenotypic correlation for yield and yield components in bread wheat. Acta Agronomica Hungarica. 2001;49(3):237–242.

Saleem U, Khaliq I, Mahmood T, Rafique M. Phenotypic and Genotypic correlation coefficient between yield and yield components in wheat. J. Agric. Res. 2006;44(1).

Misganaw Ferede, Fisseha Worede. Grain Yield Stability and Phenotypic Correlation Analysis of Bread Wheat (Triticum aestivum L.) Genotypes in North Western Ethiopia. Food Science and Quality Management. 2016;48.

Azimi Abdul Matin, Shailesh Marker, Indranil Bhattacharjee. Genotypic and phenotypic variability and correlation analysis for yield and its components in late sown wheat (Triticum aestivum L.). Journal of Pharmacognosy and Phytochemistry. 2017;6(4):167-173.

Birhanu Mecha, Sentayehu Alamerew, Alemayehu Assefa, Ermias Assefa, Dargicho Dutamo. Genotypes correlation and path coefficient studies of yield and yield associated traits in bread wheat (Triticum aestivum L.). Plants and Agriculture Research. 2017;6(5).

El-Rawy A Mahmoud, Hassan Mohamed I, Omran Mohamed F, El-defrawy Mohamed M. Gene action and combining ability of cellular Thermotolerance in bread wheat (Triticum aestivum L.). Plant Breed. Biotech. 2018;6(3):206-220.

Aliyu Usman Ibrahim: Genetic variability, Correlation and Path analysis for Yield and yield components in F6 generation of Wheat (Triticum aestivum Em. Thell.) IOSR Journal of Agriculture and Veterinary Science. 2019;17-23.

Ajmal SU, Zakir N, Mujahid MY. Estimation of Genetic Parameters and Character Association in Wheat. J. agric. biol. sci. 2009;1(1):15-18.

Dharmendra S, Singh KN. Variability analysis for yield and yield attributes of bread wheat under salt affected condition. Central Soil Salinity Research Institute, Karnal- 132001, Haryana, India; 2009.

Ghallab Samir KA Ismail, Hassan K, Abdel-Aziz Nasr Sharaan. Estimation of genotypic and phenotypic correlations for some growth traits and grain yield of Wheat genotypes. International Journal of Agronomy and Agricultural Research (IJAAR). 2017;10(5):53-58.