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Morphological traits and molecular markers analyses are very important tools for estimation of genetic variability among genotypes. In this study, we combined the phenotypic characterization with the inter-simple sequence repeats (ISSR) technique for the assessment of genetic diversity among 26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P ranged from 25 to 76.92 with an average of 46.97%. The average number of polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level of polymorphism, respectively. It is clear that the highest polymorphism level was obtained in the case of di-nucleotide repeat primers. In this work the polymorphism information content (PIC) values varied from 0.04 (UBC-819) to 0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from 0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers (UBC-811930bp and UBC-833600bp) were identified which may be linked to stem diameter and grain yield per plant traits, respectively.
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