Screening the Responsible Impact of Fourteen Bread Wheat (Triticum aestivum L.) Genotypes against Osmatic Water Stress Mediated through PEG6000 in Terms of Seed Germination and Early Seedling Growth Stage in Search of Promising Drought Tolerant Genotypes under in vitro Condition

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Radwa B. Ahmed
Aalaaa A. Aboelkassem
Shimaa M. Ali
E. Ismail
Galal A. R. El-Sherbeny
Haitham M. A. Elsayed


Seed germination is considered one of the first and critical fundamental life stage of a plant. So, the success in growth and yield production is depending on this stage. A way to overcome the negative effects of deficit water stress on plant production is development of drought tolerant genotypes. With this view an in vitro experiment was attempted to measure and quantify the negative effects of osmatic PEG6000 stress on seed germination and early seedling growth stage of fourteen wheat genotypes to determine the relevant response of studied genotypes with identifying resistant genotypes toward high stress of PEG6000. To achieve this purpose, the experiment was assigned in a split plot arrangement in two factorial CRD fashion projection with three replications under five levels of PEG6000 factors (Ψs) {0% (0 Mpa), 5% (-0.3 Mpa), 10% (-0.6 Mpa), 15% (-0.9 Mpa) and 20% (-1.2 Mpa) w/v} to establish artificial drought stress to assess the progressive effect. The observations are converted into its effects in terms of fifteen biological attributes included (germination percentage, germination index, seedling length, relative percentage of seedling length, reduction percentage of seedling length, seedling fresh weight, relative percentage of seedling fresh weight, reduction percentage of seedling fresh weight, seedling dry weight, relative percentage of seedling dry weight, reduction percentage of seedling dry weight, tissue water content, vigor index, tolerance index and coefficient of relative inhibition). The experiment was repeated for confirmation and the results were similar. The results appeared that the germination percentage was not found as effective indicator of genotypic variation for PEG6000 stress at early seedling stage. Moreover, degree of PEG6000 stress tolerance at early seedling growth stage did not maintain in all studied wheat genotypes. Therefore, the authors were done PCA plots and Euclidean distance analysis to distinguish and clear superiority PEG6000 stress-tolerant genotypes. Overall, genotypes Gimeza-12, Sakha-94, Katela, and Sids-12 are relatively drought tolerant genotypes as they showed a better performance degree of survival on high levels of induced PEG6000 and the remaining genotypes had an intermediate response toward the induced PEG6000. This method can be recommended as a tool for determining the mechanisms to the plant breeder for suitable rapid detection criteria of screening drought tolerant genotypes in a large population incorporate with the reduced cost and labor to initiate breeding programs to develop better drought tolerant genotypes as a best parents for genetic characterization through QTL analysis of mapping population derived from them.

Early seedling growth stage, Euclidean distance, germination, PEG6000, PCA plots, wheat (Triticum aestivum L.).

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B. Ahmed, R., A. Aboelkassem, A., M. Ali, S., Ismail, E., R. El-Sherbeny, G. A., & M. A. Elsayed, H. (2020). Screening the Responsible Impact of Fourteen Bread Wheat (Triticum aestivum L.) Genotypes against Osmatic Water Stress Mediated through PEG6000 in Terms of Seed Germination and Early Seedling Growth Stage in Search of Promising Drought Tolerant Genotypes under in vitro Condition. Asian Journal of Research in Biosciences, 2019(1), 78-97. Retrieved from
Original Research Article


Chachar Zaid, Chachar NA, Chachar QI, Mujtaba SM, Chachar GA, Sadaruddin Chachar. Identification of water deficit tolerant wheat genotypes under drought conditions. International Journal of Research – Granthaalayah. 2016;4(2):206-214.

Soare Marin, Paula Iancu, Elena Bonciu, Ovidiu Păniță. The effect of salicylic acid and polyetylene glycol on wheat germination. Bulletin USAMV Series Agriculture. 2018;75(1):38-43.

Ali M. Ali, Nada A. Asaad, Marwa A. Eltony, Nehal G. Abdelaziz, Omnia M. Kamal, Ahmed M. Ahmed, Galal A. R. El-Sherbieny, Haitham M. A. Elsayed. Evaluation capability of wheat (Triticum aestivum L.) genotypes under salinity (NaCl) stress as a systematic tolerance assessment at seed germination and early growth stage under laboratory conditions. Asian Journal of Research in Biosciences. 2019;1(2):65-77.

Kafi M, Salehi M. Kochia scoparia as a model plant to explore the impact of water deficit on halophytic communities. Pak. J. Bot. 2012;44:257-262.

Rana MS, Hasan MA, Bahadur MM, Islam MR. Effect of polyethylene glycol induced water stress on germination and seedling growth of wheat (Triticum aestivum L.). The Agriculturists, a Scientific Journal of Krishi Foundation. 2017;15(1):81-91.

Partheeban C, Chandrasekhar CN, Jeyakumar P, Ravikesavan R, Gnanam R. Effect of PEG induced drought stress on seed germination and seedling characters of maize (Zea mays L.) genotypes. International Journal of Current Microbiology and Applied Sciences. 2017;6(5):1095-1104.

Hubbard M, Germida J, Vujanovic V. Fungal endophytes improves wheat seed germination under heat and drought stress. Botany. 2012;90(2):137-149.

Ezzat-Ahmadi Masoud, Ahad Madani, Abdolreza Alimohammadi. Response of wheat genotypes to osmotic stress in terms of seed germination and growth of seedling. IDESIA (Chile). 2014;32(2):57-63.

Xiaoyu Li, Chunsheng Mu, Jixiang Lin. The germination and seedlings growth response of wheat and corn to drought and low temperature in spring of Northeast China. Journal of Animal &Plant Sciences. 2014;21(1):3212-3222.

Dar IA, Kamaluddin ZA Dar, Sofi PA, Lone AA. Effect of drought on the germination of maize using PEG (polyethylene glycol) as a substitute for drought screening; 2018.

Ciuca˘ M, Ba˘nica˘ C, David M, Sa˘ulescu NN. Rome Agric. Res. 2010;27:1-5.

Chaniago Irawati, Auzar Syarif, Putri Riviona. Sorghum seedling drought response: In search of tolerant genotypes. International Journal on Advanced Science Engineering Information Technology. 2017;7(3):892-897.

Pande R, Agarwal RM. Physiol. Mol. Biol. Plants. 1998;4:53-57.

Razmjoo Maryam, Reza Mohammadi, Lia Shooshtari. In vitro evaluation of durum wheat genotypes for drought tolerance. Journal on New Biological Reports. 2015;4(1):33-40.

Lu Z, Neumann PM. J. Exp. Bot. 1998;49:1945-1952.

El Siddig Marmar A, Stephen Baenziger, Ismael Dweikat, Adil A. El Hussein. Preliminary screening for water stress tolerance and genetic diversity in wheat (Triticum aestivum L.) cultivars from Sudan. Journal of Genetic Engineering and Biotechnology. 2013;11:87-94.

Hellal FA, El-Shabrawi HM, Abd El-Hady M, Khatab IA, El-Sayed SAA, Chedly Abdelly. Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. Journal of Genetic Engineering and Biotechnology. 2018;16:203–212.

Khakwani Abdul Aziz, Dennett MD, Munir M. Early growth response of six wheat varieties under artificial osmotic stress condition. Pak. J. Agri. Sci. 2011;48(2): 119-123.

Van den Berg L, Zeng Y. Response of South African indigenous grass species to drought stress induced by polyethylene glycol (PEG) 6000. South African Journal of Botany. 2006;72(2):284-286.

Ahmad S. Nariman, Shadia H. S. Kareem, Kamil M. Mustafa, Dastan A. Ahmad. Early screening of some Kurdistan wheat (Triticum aestivum L.) cultivars under drought stress. Journal of Agricultural Science. 2017;9(2):88-103.

Hamza JH. Seed priming of bread wheat to improve germination under drought stress. Iraqi Journal of Agricultural Sciences. 2012;43(2):100-107.

Qayyum A, Razzaq A, Ahmad M, Jenks MA. Water stress causes differential effects on germination indices, total soluble sugar and proline content in wheat (Triticum aestivum L.) genotypes. African Journal of Biotechnology. 2011;10(64): 14038-14045.

Govindaraj M, Shanmugasundaram P, Sumathi P, Muthiah AR. Electron. J. Plant Breeding. 2010;1:590-599.

Rauf M, Munir M, Hassan M, Ahmad M, Afzal M. Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. Afr. J. Biotech. 2006;6:971-975.

Noorka IR, Khaliq I. An efficient technique for screening wheat (Triticum aestivum L.) germplasm for water deficit tolerance. Pak. J. Bot. 2007;39(5):1539-1546.

Islam MM, Kayesh E, Zaman E, Urmi TA, Haque MM. Evaluation of rice (Oryza sativa L.) genotypes for drought tolerance at germination and early seedling stage. The Agriculturists. 2018;16(1):44-54.

Hakizimana F, Haley SD, Turnipseed EB. Repeatability and genotype x environment interaction of coleoptile length measurements in winter wheat. Crop Science. 2002;40:1233-1237.

Farshadfar Ezatollah, Parvin Elyasi, Shokouh Dabiri. Association between in vitro and in vivo predictors of drought tolerance in the landraces of bread wheat (Triticum aestivum L.). European Journal of Experimental Biology. 2012;2(4):984-994.

Španić Valentina, Maja Ižaković, Tihana Marček. Wheat germination and seedlings under peg-induced conditions. Agronomski Glasnik. 2017;3. ISSN: 0002-1954.

Muscoloa Adele, Maria Sidaria, Umberto Anastasib, Carmelo Santonocetoa, Albino Maggio. Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions. 2014;9(1):354-363.

Abdul-Baki AA, Anderson JD. Viability and leaching of sugars from germinating barley. Crop Sci. 1970;10:31-34.

Maiti RK, Vidyasagar P, Rajkumar D, Ramaswamy A, Rodriguez HG. Seed priming improves seedling vigor and yield of few vegetable crops. Int. J. Bio-res. Stress Manag. 2011;2(1):125- 130.

Mercado A. Structure and function of plants in saline habitats: New trends in study of salt tolerance (Translation by Golleck, N.) John Willey and Sons, New York, USA. 1973;160-196.

Srividhya Akkareddy, Lakshminarayana R. Vemireddy, Puram Venkata Ramanarao, Sakile Sridhar, Mudduluru Jayaprada, Ghanta Anuradha, Battiprolu Srilakshmi, Hariprasad K. Reddy, Arramsetty Subramanyam Hariprasad, Ebrahimali Abubackar Siddiq. Molecular mapping of QTLs for drought related traits at seedling stage under PEG induced stress conditions in rice. American Journal of Plant Sciences. 2011;2:190-201.

Yan W, Kang MS. Biplot analysis: A graphical tool for breeders, geneticists and agronomist, CRC Press, Boca Raton, FL. 2003;313.

Taherkhani Tofigh, Navid Rahmani, Amin Moradi Aghdam, Peiman Zandi. Assessment of nitrogen levels on flower yield of calendula grown under different water deficit stresses using drought tolerant indices. Journal of American Science. 2011;7(10):591-598.

Shaban M. Effect of water and temperature on seed germination and emergence as a seed hydrothermal time model. International Journal of Advanced Biological and Biomedical Research. 2013;1(12):1686-169.

Wuest SB, Albrecht SL, Skirvin KW. Vapor transport vs. seed- soil contact in wheat germination. Agronomy Journal. 1999;91: 783-787.

Mohammadi N, Mojaddam M. The effect of water deficit stress on germination components of grain sorghum cultivars. Indian Journal of Fundamental and Applied Life Sciences. 2014;4(4):289-291.

Soltani A, Galeshi S, Zeinali E, Latifi N. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Science and Technology. 2002;30:51-60.

Khan M, Shabbir G, Akram Z, Shah M, Ansar M, Cheema N, Iqbal M. Character association studies of seedling traits in different wheat genotypes under moisture stress conditions. SABRAO Journal of Breeding and Genetics. 2013;45(3):458-467.

Yoshimura K, Masuda A, Kuwano M, Yokota A, Akashi K. Programmed proteome response for drought avoidance/tolerance in the root of a C3 xerophyte (wild watermelon) under water deficits. Plant and Cell Physiology. 2008;49(2):226-241.

Pratap V, Sharma YK. Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo). Journal of Environmental Biology. 2010;31(5):721-726.

Harb MA. Reserve mobilization, total sugars and proteins in germinating seeds of durum wheat (Triticum durum Desf.) under water deficit after short period of imbibition. Jordan Journal of Biological Sciences. 2013;6(1):67-72.

Biesaga-Kościelniak J, Ostrowska A, Filek M, Dziurka M, Waligórski P, Mirek M, Kościelniak J. Evaluation of spring wheat (20 varieties) adaptation to soil drought during seedlings growth stage. Agriculture. 2014;4(2):96-112.

Martins CC, Martinelli-Seneme A, Castro MM, Nakagawa J, Cavariani C. Comparação entre métodos para a avaliação do vigor de lotes de sementes de couve-brócolos (Brassica oleracea L. var. Italica Plenk). Revista Brasileira de Sementes. 2002;24:96-101.

McDonald MB. Seed priming. Black M, Bewley JD, (Eds.), Seed Technology and Its Biological Basis, Sheffield Academic Press, Sheffield, UK. 2000;287–325.

Sadeghi H, Khazaei F, Yari L, Sheidaei S. Effect of seed osmopriming on seed germination behavior and vigor of soybean (Glycine max L.). J. Agric. Biol. Sci. 2011;6(1):39-43.

Mollasadeghi V, Ghanifathi T, Masoumzadeh B, Aghahasanbeyglo AA. Bread wheat tolerance against drought at early growth stages and grain filling period. Applied Mathematics in Engineering, Management and Technology. 2014;2(2): 50-59.

Bayoumi TY, Manal HE, Metwali EM. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. Afr. J. Biotechnol. 2008;7: 2341–2352.

Gholamin R, Khayatnezhad M. Effects of polyethylene glycol and NaCl stress on two cultivars of wheat (Triticum durum) at germination and early seeding stages. American – Eurasian J. Agric. Environ. Sci. 2010;9(1):86-90.

Datta J, Mondal T, Banerjee A, Mondal N. Assessment of drought tolerance of selected wheat cultivars under laboratory condition. Journal of Agricultural Science and Technology. 2011;7:383-393.

Marcin´ska Izabela, Ilona Czyczyło-Mysza, Edyta Skrzypek, Maria Filek, Stanisław Grzesiak, Maciej T. Grzesiak, Franciszek Janowiak, Tomasz Hura, Michał Dziurka, Kinga Dziurka, Agata Nowakowska, Steve A. Quarrie. Impact of osmotic stress on physiological and biochemical characteristics in drought-susceptible and drought-resistant wheat genotypes. Acta Physiol Plant. 2013;35:451-461.
DOI: 10.1007/s11738-012-1088-6

Machado FHB, David AMS de S, Cangussú LVS, Figueiredo JC, Amaro HTR. Physiological quality of seed and seedling performance of crambe genotypes under water stress. Revista Brasileira de Engenharia Agrícola e Ambiental. 2017;21(3):175-179.

Sheshaiah Shankergoud I, Siddhesh Nadkarni R, Vikas V. Kulakarni. Screening of sunflower (Helianthus annuus L) genotypes for moisture stress tolerance using PEG-6000. International Journal of Current Microbiology and Applied Sciences. 2017;6(5):848-856.

Kayacetin Fatma, Banu Efeoglu, Behrouz Alizadeh. Effect of NaCl and PEG-induced osmotic stress on germination and seedling growth properties in wild mustard (Sinapis arvensis L.). Anadolu, J. of Aari. 2018;28(1):62-68.

Farsiani A, Ghobadi ME. Effects of PEG and NaCl stress on two cultivars of corn (Zea mays L.) at germination and early seedling stages. World Acad. Sci. Eng. Tech. 2009;57:382-85.

Khayatnezhad M, Gholamin R, Jamaatie-Somarin SH, Zabihi-Mahmoodabad R. Effects of peg stress on corn cultivars (Zea mays L.) at germination stage. World Appl. Sci. J. 2010;11(5):504-506.

Mostafavi KH. An evaluation of safflower genotypes (Carthamus tinctorius L.), seed germination and seedling characters in salt stress conditions. Afr. J. Agric. Res. 2011;6(7):1667-1672.

Singh AK, Prakash V, Sastry EVD. Effect of salinity stress on seed germination and seeding growth of wheat. Agric. Sci. Digestion. 2000;20(2):96-98.

Moud AM, Maghsoudi K. Salt stress effects on respiration and growth of germinated seeds of different wheat (Triticum aestivum L.) cultivars. World J. Agril. Sci. 2008;4(3): 351-358.

Baque A, Nahar M, Yeasmin M, Quamruzzaman M, Rahman A, Azad MJ, Biswas PK. Germination behavior of wheat (Triticum aestivum L.) as influenced by polyethylene glycol (PEG). Universal J. Agril. Res. 2016;4(3):86-91.

Baloch MJ, Dunwell J, Khakwani AA, Dennett M, Jatoi WA, Channa SA. Assessment of wheat cultivars for drought tolerance via osmotic stress imposed at early seedling growth stages. Journal of Agricultural Research. 2012;50(3):299-310.

Chachar M, Chachar N, Chachar S, Chachar Q, Mujtaba S, Yousafzai A. In-vitro screening technique for drought tolerance of wheat (Triticum aestivum L.) genotypes at early seedling stage. Journal of Agricultural Technology. 2014;10(6): 1439-1450.

Hassan MI, Mohamed EA, El-Rawy MA, Amein KA. Evaluating inter specific wheat hybrids based on heat and drought stress tolerance. Journal of Crop Science and Biotechnology. 2016;19(1):85-98.

Mujtaba SM, Summiya F, Khan MA, Mumtaz A, Barakat K. Physiological studies on six wheat (Triticum aestivum L.) genotypes for drought stress tolerance at seedling stage. Agricultural Research and Technology: Open Access Journal. 2016;1(2):1-6.

Fraser T, Silk W, Rosr T. Effect of low water potential on cortical cell length in growing region on maize roots. Plant Physiology. 1990;93:648-651.

Kamran M, Muhammad S, Muhammad A, Nudrat A. Aisha. Alleviation of drought-induced adverse effects in spring wheat (Triticum aestivum L.) using proline as a pre-sowing seed treatment. Pak. J. Bot. 2009;41(2):621-632.

Moucheshi A, Heidari B, Assad M. Alleviation of drought stress effects on wheat using arbuscular mycorrhizal symbiosis. International Journal of Agriculture Science. 2012;2(1):35-47.

Ming DF, Pei ZF, Naeem MS, Gong HJ, Zhan WJ. Silicon alleviates PEG- induced water-deficit stress in upland rice seedling by enhancing osmotic adjustment. Journal of Agronomy and Crop Science. 2012;198: 14-26.

Steven R. Becker, Patrick F. Byrne, Scott D. Reid, William L. Bauerle, John K. McKay, Scott D. Haley. Root traits contributing to drought tolerance of synthetic hexaploid wheat in a greenhouse study. Euphytica. 2016;207:213–224.

Ahmad M, Shabbir G, Minhas NM, Shah MKN. Identification of drought tolerant wheat genotypes based on seedling traits. Sarhad J. Agric. 2013;29(1):21-27.

Meiri A, Poljakoff-Mayber A. Effect of various salinity regimes on growth, leaf expansion and transpiration rate of bean plants. Soil Sci. 1970;109:26-39.

Farshadfar E, Kianifar S, Chaghakabodi R. GT biplot analysis of genetic diversity in bread wheat using in vitro indicators of drought tolerance, Int. J. Biol. Sci. 2015;7:1439-1447.

Golabadi M, Arzani A, Maibody SM. Assessment of drought tolerance in segregating populations in durum wheat. African Journal of Agricultural Research. 2006;1(5):162-171.

El-Mohsen AAA, El-Shafi MA, Gheith E, Suleiman H. Using different statistical procedures for evaluating drought tolerance indices of bread wheat genotypes. Advance in Agriculture and Biology. 2015;4(1):19-30.

Jaijarmi V. Effect of water stress on germination indices in seven wheat cultivar. World Academy of Science Eng. Technol. 2009;49:105-106.

Alaei M, Zaefizadeh M, Khayatnezhad M, Alaei Z, Alaei Y. Evaluation of germination properties of different durum wheat genotypes under osmotic stress. Middle-East Journal of Scientific Research. 2010;6:642646.

Abdi H, Williams LJ. Principal component analysis. Wiley Interdisciplinary Reviews: Computational Statistics. 2010;2(4):433-459.

Farshadfar E, Jamshidi B, Chehri M. Assessment of immature embryo culture to select for drought tolerance in bread wheat. Int. J. Bio-Sci. 2014;4(4):194- 203.

Farshadfar E, Amiri R. In vitro application of integrated selection index for screening drought tolerant genotypes in common wheat. Acta Agric. Slovenica. 2016;107: 335-344.

Kacem Nadia Sandra, Fabienne Delporte, Yordan Muhovski, Abdelhamid Djekoun, Bernard Watillon. In vitro screening of durum wheat against water stress mediated through polyethylene glycol. Journal of Genetic Engineering and Biotechnology. 2017;15:239–247.

Salim MH, Todd GW, Stutte CA. Evaluation of techniques for measuring drought avoidance in cereal seedlings. Agron. J. 1969;61:182-185.

Sammons DJ, Peters DB, Hymowitz T. Screening soybeans for drought tolerance. I. Growth chamber procedure. Crop Sci. 1978;18:1050-1055.

Boubaker M, Yamada T. Differential genotypic responses of string wheat early growth to limited moisture conditions. Tropicultura. 1995;13(2):50-53.