Comparative Study for Bioethanol Production from Waste Paper Using E. coli and S. cerevisiae Specie

PDF Review History

Published: 2024-03-27

Page: 38-48

Zeynu Shamil Awol *

Department of Chemical Engineering, Wolkite University Engineering and Technology College (CET), Wolkite, Ethiopia.

Rezika Tofike Abate

Department of Chemical Engineering, Wolkite University Engineering and Technology College (CET), Wolkite, Ethiopia.

*Author to whom correspondence should be addressed.


Biomass energy is a sustainable energy source derived from plant and animal material. Bio-ethanol, bio-methanol, and biodiesel are examples of biomass fuels. One of the most significant alternative energy sources that replaces fossil fuels is bio-ethanol. The goal of this study is to create bio-ethanol from waste workplace paper. It was then subjected to weak acid hydrolysis. For each row materials such as sugarcane, A4 paper, fruit peel and hard paper after those diluted acid hydrolysis process this analysis of study validate that (99.8,99.1and 96.3) mg/g glucose content extracted from sugarcane, A4 paper, fruit peel and hard paper respectively with 1:85 (g/ml) of solid-liquid ratio. those extracted glucose content were also subjected to two different fermentation process by using E. coli and S. centeriease microbial strain followed by distillation for further processing the result analyzed by design expert software  file version, study type response surface, design type Box-Bohnken and design model quadratics by three factors and levels with one response factors(alcohol content in %) then 17 runs were conducted for each microbial strain of experiments then the analysis attested or significant quantity of bio-ethanol was detected that 80.9% alcohol content obtained from 120 hr, 36°c, 5 and 36 hr, 36°c, 5 time temperature and PH value respectively by using E. coli and with other scenario by using  S. centriease 97% of alcohol content obtained by 36hr, 47°c and 6.5 time temperature and PH value respectively. Therefore obesely bioethanol is cost benefit energy source so any body should be apply in the field and home level users too.

Keywords: Wastepaper, hydrolysis, fermentation, distillation and bio-ethanol, filteration, centrifugation

How to Cite

Awol , Z. S., & Abate , R. T. (2024). Comparative Study for Bioethanol Production from Waste Paper Using E. coli and S. cerevisiae Specie. Asian Journal of Research in Biosciences, 6(1), 38–48. Retrieved from


Download data is not yet available.


Solomon BD, et al. Grain and cellulosic ethanol: History, economics, and energy policy. Biomass & Bioenergy. 2007;31:416-425.

Stokes H. Alcohol fuels (ethanol and methanol): safety. In: Presentation at ETHOS conference, Seattle, Washington; 2005.

Galbe M, Zacchi G. A review of the production of ethanol from softwood. Appl. Biochem. Biotechnol. 2007;59:618-628.

Meher LC, Sagar DV, Naik SN. Technical aspects of biodiesel production by trans-esterification—A review Renew Sustain Energy Rev. 2006;248–268.

Balat H. Prospects of biofuels for a sustainable energy future: A critical assessment. Energy Educ Sci Technol Part A. 2010;24:85–11.

Kim SH. Lime pretreatment and enzymatic hydrolysis of corn stover. Doctoral dissertation, Texas A&M University; 2004.

Lark N, Xia Y, Qin C-G, Gong CS, Tsao GT. Production of ethanol from recycled paper sludge using cellulase and yeast, Biomass Bioenergy. 1997;12:135–43.

Lee D, Owens VN, Boe A, Jeranyama P. Composition of herbaceous biomass feedstocks. South Dakota State University Publication, SGINC1-07, Brookings, SD; 2007.

Wang L, et al. Applied Energy. 2012;99: 23–31.

Ministry of Finance and Economic Development (MoFED). Ethiopia: Country Report on the Implementation of the Brussels Program of Action (BPOA). Addis Ababa; 2010.

Steinbach V, Wellmer FW. Consumption and Use of Non-Renewable Mineral and Energy Raw Materials from an Economic Geology Point of View. Sustainability. 2010;2(5):1408-1430.

Balat M, Balat H, Oz C. Progress in bioethanol processing. Progr Energy Combust Sci. 2008;34:551–73.

Cardona C, Sanchez O. Fuel ethanol production: Process design trends and integration opportunities. Biores. Technol. 2007;98:2415–2457.

Cunningham HW. Non-heatset Web Printing. Draft of Document AP 40, Washington, D.C.: American Newspaper Publishers Association; 1990.

Demirbas A. Bioethanol from Cellulosic Materials: A Renewable Motor Fuel from Biomass. Energy Sources. 2005;27:327-337.

Gaur K. Process optimization for the production of ethanol via fermentation. Master thesis, Thapar Institute of Engineering and Technology, Patiala; 2006.

Molina-Sabio M, Rodríguez-Reinoso F. Role of chemical activation in the development of carbon porosity. Colloid Surface Physico-chemEng Aspect. 2004; 15–25.

Madson P, Lococo D. Recovery of volatile products from dilute highfouling process streams. Appl. Biochem. Biotechnol. 2000; 84–86:1049–1061.

Hamelinck C, van Hooijdonk G, Faaij A. Prospects for ethanol from lignocellulosic biomass: Techno-economic performance as development progresses, scientific report- NWS-E-2003-55. Utrecht University, Utrecht, the Netherlands: Copernicus Institute, Department of Science, Technology and Society. 2003; 35.

Hopkins WG. Introduction to Plant Physiology, 2nd edition. John Wiley & Sons, Inc., New York; 2008.

Ikeda Y, Park EY, Naoyuki O. Bioconversion of waste office: Bioresource Technology. 2006;97:1030–1035.

Karuppiah R, Peschel A, Martin M, Grossmann I, Zullo L. Special Symposium-EPIC-1: European Process Intensification Conference—1, Copenhagen; 2007.

Miles WE, et al., eds. Pulp and Paper 1988 Fact Book. San Francisco, Calif.: Miller Freeman Publications Inc; 1988.

McMillan J. Pre-Treatment of Lignocelluloses Biomass; 1994.

Sun Y, Cheng J. Hydrolysis of lignocellulosic materials for ethanol production: A review. Biores. Technol. 2002;83:1-11.

Ulrich G. The Fate and Transport of Ethanol-Blended Gasoline in the Environment.-Submitted by Governeur‘s Ethanol Coalition; Lincoln, Nebrasa. 1999;103.

Wayman M, Parekh SR. Bioiechnolovy of Biomasa Conversion: Fuels and Cliemn ills Iront Renc Mible Resources. Wiley UK and Prenlice Hall. LS.A.; 1990.

WWI (WORLD WATCH INSTITUTE). Biofuels for Transportation, Global Potential and Implications for Agricultural and Energy in the 21st Century; 2006.

Yoosin S, Sorapipatana C. A Study of ethanol production cost for gasoline substitution in Thailand. Thammasat. Int. J. Sci. Technol. 2007;12:69–80.