Response of Some Microorganisms, Earthworms and Snails to Pesticides (Carbofuran and Paraquat) under Tropical Conditions

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Published: 2024-03-19

Page: 17-31


Ataikiru T. L. *

Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria.

Okorhi F. B.

Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Aim: to determine the response of microorganisms, earthworms and snails to pesticides under tropical conditions.

Study Design: soil experiment with a controlled lighting system of 12 hour of light and 12 hour of darkness was adopted.

Place and Duration of Study: Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Nigeria/three months.

Methodology: Acute effect of pesticides on earthworms and snails was assessed using a 14 day soil bioassay test as recommended by Organization for Economic Cooperation and Development (OECD) and International Organization for Standardization (ISO). Effect of pesticides application on microorganisms in soil was evaluated via standard microbial counts.

Results: Toxicity of Carbofuran to the earthworms and snails was higher relative to Paraquat with a median lethal concentration (LC50) of 10.7 mg/kg; 159571 mg/kg (earthworms) and 23.22 mg/kg; 759000mg/kg (snails), respectively. Microbial counts increased in pesticides treated soil after the initial decline in numbers. Total heterotrophic bacterial counts in Carbofuran and Paraquat treated soils increased from 1.59 × 106 CFU/g to 2.41 × 106 CFU/g and 1.46 × 106 CFU/g to 2.08 × 106 CFU/g, respectively for day 14 to 21. A reverse trend was observed in control soil. Fungal counts increased in Carbofuran (1.18 × 105 CFU/g to 1.42 × 105 CFU/g); Paraquat (1.53 × 105 CFU/g to 1.79 × 105 CFU/g) from day 14 to day 21. Actinomycetes counts were in magnitude of 104 CFU/g soil for both Carbofuran and Paraquat treated soils, while population of phosphate solubilizers and nitrifiers were in 105 CFU/g soil.

Conclusion: Toxicity test estimates the possibility that antagonistic environmental impacts/influence might take place or are taking place due to exposure to sole or additional pesticides. Pesticides had no adverse effects on the microorganisms at recommended field rates. Thus, their use must strictly be based on these recommended rates.

Keywords: lethal toxicity, pesticides, microorganisms, snails, earthworms, tropics


How to Cite

Ataikiru T. L., & Okorhi F. B. (2024). Response of Some Microorganisms, Earthworms and Snails to Pesticides (Carbofuran and Paraquat) under Tropical Conditions . Asian Journal of Research in Biosciences, 6(1), 17–31. Retrieved from https://globalpresshub.com/index.php/AJORIB/article/view/1978

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References

Wood TJ, Goulson D. The environmental risks of neonicotinoid pesticides: a review of the evidence post 2013. Environ. Sci. Pollut. Res. 2017;24:17285–17325.

Dar MA, Baba ZA, Kaushik G. A review on phorate persistence, toxicity and remediation by bacterial communities. Pedosphere. 2022;32(1):171-183.

Joko T, Dewanti NAY, Dangiran HL. Pesticide Poisoning and the use of personal protective equipment (PPE) in Indonesian farmers. Journal of Environmental and Public Health. 2020; 1-7.

Phaceli A, Kone M, Dembele A, Haba J, Yao E. Effect of Herbicides Used in Horticulture (2,4-D, Glyphosate and Nicosulfuron) on Snails Achatina fulica (Bowdich, 1720). Journal of Agricultural Chemistry and Environment. 2021;10: 402-414.

Bonvoisin T, Utyasheva L, Knipe D, Gunnel D, Eddleston M. Suicide by Pesticide Poisoning in India: A Review of Pesticide Regulations and their Impact on Suicide Trends. BMC Public Health. 2020;20:251.

Mohring N, Ingold K, Kudsk P, Martin-Laurent F, Niggli U, Siegrist M, Studer B, Walter A, Finger R. Pathways for advancing pesticide policies. Nature Food. 2020;1(9):535–540.

Sarwar M. The killer chemicals for control of agriculture insect pests: The botanical insecticides. International Journal of Chemical and Biomolecular Science. 2015;1:123–128.

Ojo MO, Zahid A. Deep learning in controlled environment agriculture: a review of recent advancements, challenges and prospects. Sensors. 2022; 22:7965.

Dar MA, Kaushik G, FranciscoJ, Chiu V. Pollution status and biodegradation of organophosphate pesticides in the environment, Editor(s): Pardeep Singh, Ajay Kumar, Anwesha Borthakur, Abatement of Environmental Pollutants, Elsevier. 2020;25-66.

Benicha M, Mrabet R, Azmani A. Dissipation processes of 14C-carbofuran in soil from Northwest Morocco as influenced by soil water content, temperature and microbial activity. Journal of Environmental Chemistry and Ecotoxicology. 2013;5(5):119-128.

Palaniyappan J, Venugopal D, Duraisamy E, Beerappa R. Pesticides and human health implications, In (eds): Mohammad Hadi Dehghani, Rama Rao Karri, Ioannis Anastopoulos, Pesticides Remediation Technologies from Water and Wastewater, Elsevier. 2022;3-21.

Dey D. Impact of indiscriminate use of insecticide on environmental pollution. Internat. J. Plant Protec. 2016;9(1):264-267.

Moretti ML, Shrestha A, Hembree KJ, Hanson BD. Post emergence control of glyphosate/paraquat-resistant hairy fleabane (Conyza bonariensis) in tree nut orchards in the Central Valley of California. Weed Technology. 2015;29 (3):501–508.

Hawkes TR. Mechanisms of resistance to paraquat in plants. Pest Management Science. 2014;70(9):1316–1323

Tudararo-Aherobo LE, Okorhi- Damisa FB. Comparative study of metagenomic profile of bacteria strains present in an abandoned artisanal refinery site in Obi-Ayagha Community, Delta State, Nigeria. Journal of Applied life Sciences International. 2023a;26(3):30-40.

Ogeleka DF, Onwuemene CJ, Okieimen FE. Toxicity potential of Grassate a non-selective herbicides on snails (Achachatina marginata) and earthworms (Aporrectodea longa). Chemistry and Ecology. 2017; 33(5):447- 463.

Keer JT, Birch L, Molecular methods for the assessment of bacterial viability. Journal of Microbiological Methods. 2013;53:175-183.

Alves PRL, Cardoso EJBN, Martines AM, Sousa JP. Earthworm ecotoxicological assessment of pesticides used to treat seeds under tropical conditions. Chemosphere. 2013;90:2674-2682.

Ahmed ST. The Impact of four pesticides on the earthworm (Lumbricus terrestris). International Journal of Current Research and Review. 2013;5(21):01-05.

Yuguda AU, Abubakar Z A, Jibo AU, Abdulhameed A, Nayaya AJ. Assesment of toxicity of some agricultural pesticides on earthworm (Lumbricus terrestris). American-Eurasian Journal of Sustainable Agriculture. 2015;9(4):49-59.

Miglani R, Bisht SS. World of earthworms with pesticides and insecticides. Interdisciplinary Toxicology. 2019; 12(2): 71–82

Patel P, Prajapati R. Possible toxicological effect of pesticides, cypermethrin on earthworm (Eisenia fetida). International Journal of Research in Engineering, Science and Management. 2020;3(12):138-143.

Ibrahim AM, Hussein AAA. Toxicological impact of organophosphorus Chlorpyrifos 48%EC pesticide on hemocytes, biochemical disruption, and molecular changes in Biomphalaria alexandrina snails. Pesticide Biochemistry and Physiology. 2022;186:105154.

Berrouk H, Necib A, Hamaizia Y, Chabi, C-B, Hmaidia K. Acute Toxicity of an insecticide (Acetamiprid) on Lumbricus Terrestris. Animal Research International. 2023;20(1):4726–4733.

Gunstone T, Cornelisse T, Klein K, Dubey A, Donley N. Pesticides and Soil Invertebrates: A Hazard Assessment. Front. Environ. Sci. 2021;9:643847.

Bart S, Laurent C, Pery ARR, Mougin C, Pelosi C. Differences in sensitivity between earthworms and enchytraeids exposed to two commercial fungicides. Ecotoxicol. Environ. Saf. 2017;140:177–184.

Alves PRL, Elke CN, Cardoso JBN, Martinesa AM, Sousa JP, Pasinic A. Seed dressing pesticides on springtails in two ecotoxicological laboratory tests. Ecotoxicology and Environmental Safety. 2014;105:65-71.

Wang K, Pang S, Mu X, Qi S, Li D, Cui F, Wang C. Biological response of earthworm, Eisenia fetida, to five neonicotinoid insecticides. Chemosphere. 2015;132:120–126.

Bhandari G, Atreya K, Scheepers PT, Geissen V. Concentration and distribution of pesticide residues in soil: non-dietary human health risk assessment. Chemosphere. 2020;253:126594.

Rico A, Sabater C, Castillo MA. Lethal and sub-lethal effects of five pesticides used in rice farming on the earthworm Eisenia fetida. Ecotoxicol and Environ Saf. 2016;127:222–229.

Bart S, Amosse J, Lowe CN, Mougin C, Pery ARR, Pelosi C. Aporrectodea caliginosa, a relevant earthworm species for a posteriori pesticide risk assessment: current knowledge and recommendations for culture and experimental design. Environ. Sci. Pollut. Res. Int. 2018; 25:33867–33881.

Jeyanthi V, Paul JAJ, Selvi BK, Karmegam N. Comparative Study of Biochemical Responses in Three Species of Earthworms Exposed to Pesticide and Metal Contaminated Soil. Environ Process. 2016;3:167–178.

Gouissi FM, Adimi AE. Bioavailability of Agricultural Pesticides on the Land Snails in the Commune of Kérou in the North of Benin. Open Journal of Soil Science. 2019;9(7):

Wang G, Shi, H, Du, Z, Chen H, Peng J, Gao S. Bioaccumulation mechanism of organophosphate esters in adult zebrafish (Danio rerio). Environmental Pollution. 2017;229:177 –187.

International Organization for Standardization (ISO). Protocol for testing soil quality #15952 – effects of pollutants on juvenile land snails (Helicidae) – determination of the effects on growth by soil contamination. Paris; 2006.

Organization for economic cooperation and development (OECD). Earthworm, acute toxicity test. OECD guideline for testing chemicals 207.Paris: OECD: 1984;1-9.

Wibawa W, Mohamad RB, Puteh AB, Omar D, Juraimi AS, Abdullah SA. Residual phytotoxicity effects of paraquat, glyphosate and glufosinate ammonium herbicides in soils from field- treated plots. International Journal of Agriculture and Biology; 2009;11:214-216.

Baboo M, Pasayat M, Samal A, Kujur M, Maharana JK, Pate AK. Effect of four herbicides on soil organic carbon, microbial biomass- C, enzyme activity and microbial populations in agricultural soil. International Journal of Research in Environmental Science and Technology. 2013;3(4):100-112.

Lone AH, Raverkar KP, Pareek N. In-vitro effects of herbicides on soil microbial communities. The Bioscan. 2014;9(1):11-16.

Ataikiru TL, Okorhi-Damisa FB. Biodegradation of carbofuran and paraquat by indigenous soil microorganisms. Journal of Advances in Biology & Biotechnology. 2022;25(10):24-34.

Alharbi SA, Arunachalam C, Murugan AM, Wainwright M. Antibacterial activity of actinomycetes isolated from terrestrial soil of Saudi Arabia. Journal of Food, Agriculture and Environment. 2012; 10(2): 1093-1097.

Organization for Economic Co-operation and Development (OECD). Environment, health and safety publication series on pesticides persistent, bioaccumulative and toxic pesticides in OECD member countries result of survey on data requirements and risk assessment approaches. 2003;15:1-67.

Lima MP, Cardoso DN, Soares AM, Loureiro S. Carbaryl toxicity prediction to soil organisms under high and low-temperature regimes. Ecotoxicol Environ Saf. 2015;114:263–27.

Arimoro F. Heavy metal content in the African giant snail Achachatina marginata in Southern Nigeria. Folia Malacologica. 2008;16(1):31-34.

Diez MC, Elgueta S, Rubilar O, Tortella GR, Schalchli H, Bornhardt C, et al. Pesticide dissipation and microbial community changes in a biopurination system: influence of the rhizosphere. Biodegradation. 2017;28:395-412.

Tudararo-Aherobo LE, Ataikiru TL. Effects of chronic use of herbicides on soil physicochemical and microbiological characteristics. Microbiology Research Journal International. 2020;30(5):9-19.

Ławniczak Ł, Wo´zniak-Karczewska M, Loibner AP, Heipieper HJ, Chrzanowski L. Microbial degradation of hydrocarbons: Basic principles for bioremediation: A review. Molecules. 2020;25(4):856.

Boto ML, Magalthaes C, Perdigao R. Harnessing the potential of Native Microbial Communities for Bioremediation of oil spills in the Iberian Peninsula NW Coast. Frontiers in Microbiology. 2021;12: 1633-1659.

Jones AM, James II, Akpan PS, Eka II, Oruk AE, Ibuot AA. Characterization of Hydrocarbon Utilizing Bacteria in waste engine oil impacted sites; 2021.

Tudararo-Aherobo LE, Okorhi- Damisa FB. Effects of biostimulation and bioaugmentation on microbial growth and bioremediation of hydrocarbon contaminated soils. International Journal of Scientific Development and Research. 2023b;8(3):

Okorhi-Damisa FB, Tudararo-Aherobo LE. Monitoring the degradation of total petroleum hydrocarbon in hydrocarbon impacted site using a consortium of hydrocarbon utilizing bacteria. Journal of Advances in Microbiology Research. 2023;4(1):123-127.

Vandana LJ, Rao PC, Padmaja G. Effect of herbicides and nutrient management on soil enzyme activity. Journal of Rice Resources. 2012;5:1-12.

De-Silver SFC, Gomed de Almeida NMP, da Rocha e Silva S. Soil remediation: An overview of technologies and trends. Energies. 2020;13(18):4664.

Khalid S, Baloo L, Kumar SN. Bioremediation of Total Petroleum Hydrocarbons (TPH) by Bioaugmentation and Biostimulation in Water with Floating Oil Spill Containment Booms as Bioreactor Basin. International Journal of Environmental Research and Public Health; 2021.

Kopittke PM, Enzo L. Soil and the intensification of agriculture for global security, Science Direct on Environment International. 2019;132(5):1050-1078.

Zain MMM, Mohamad RB, Sijam K, Morshed MM, Awang Y. Effects of selected herbicides on soil microbial populations in oil palm plantation of Malaysia: A microcosom experiment. African Journal of Microbiologiy and Resources. 2013;7(5): 367-374.

Fenner K, Canonica S, Wackett LP, Eisner M. Evaluating pesticide degradation in the environment: blind spots and emerging opportunities. Science. 2013;341(6147): 752–758.

Vischetti C, Casucci C, De Erardi A, Monaci E, Tiano L, Marcheggiani F, Ciani M, Comitina F, Marini E, Taskin E, Puglisi E. Sub-lethal effects of pesticides on the DNA of soil organisms as early ecotoxicological biomarkers. Frontiers in Microbiology. 2020;11:1892.

Karpouzas DG, Vryzas Z, Marti-Lauret F. Pesticide soil microbial toxicity: setting the scee for a new pesticide risk assessment for soil microorganisms (IUPAC Technical Report). Pure and Applied Chemistry. 2022;94(10):1161-1194.

Jiang YM, Lin D, Guan XJ, Wang JF, Cao GP, Zhu D et al. Effects of herbicide used with years (8+1) on soil enzymic activity and microbial population diversity. J. Soils Sediments. 2017;17:2490-2499.