Desiccation and Freezing Sensitivity of Selected Groundnut Genotypes for Germplasm Conservation

Main Article Content

Entisar Elilah, B. Mohammed Ahmed
Faisal, E. Ahmed
Makeen, A. Makeen
Salah Eldeen, E. Ahmed

Abstract

The Series of experiments were carried out to determined the sensitivity of five groundnut genotypes  which were:  Sodari, Ghibaish, Barberton, ICGV121 and Advance  to desiccation under 7 saturated salt solutions with specific relative humidity at 35ºC and freezing in liquid nitrogen temperature                  (-196ºC) using desorption drying method. The objectives of the study were to quantify seed desiccation and freezing sensitivity and their relations to seed viability. The equilibrium seed water content of desiccated seeds, germination percentages and rates of desiccated and desiccated-frozen seeds, WC50, water activity, water potential, HMFL and their correlations with other parameters were determined. Equilibrium mean water content in gH2Og-1dw was determined gravimetrically when seeds were oven-dried under 105ºC for 24 hrs. Seed desiccation sensitivity, (designated as WC50) was obtained using the Quantal Quadric response model. Freezing sensitivity was determined using high moisture freezing limit (HMFL). The significant increases in seed viability (germination %) with decreases in equilibrium water content observed in this study. All seeds withstand low level of equilibrium water content (below 0.1 gH2Og-1dw) implying that, there were not sensitive to desiccation and freezing as determined by sensitivity indicators (WC50, wa50, Ψ50 and HMFL). In contrast, Barberton, Ghibaish and the Advance line showed less sensitivity to liquid nitrogen (HMFL value of 0.1 gH2Og-1dw) compared to Sodari and ICGV121 (HMFL values of 0.06 and 0.05 gH2Og-1dw, respectively). Correlation analyses indicated that, equilibrium seed water content was positively correlated with seed germination after desiccation and freezing. WC50 was positively correlated with HMFL indicating that the sensitivity of seeds to freezing depends upon its level of desiccation.

Keywords:
Desiccation, freezing, conservation, groundnut, genotypes

Article Details

How to Cite
Ahmed, E. E. B. M., Ahmed, F. E., Makeen, M. A., & Ahmed, S. E. E. (2020). Desiccation and Freezing Sensitivity of Selected Groundnut Genotypes for Germplasm Conservation. Asian Basic and Applied Research Journal, 2(1), 26-39. Retrieved from https://globalpresshub.com/index.php/ABAARJ/article/view/843
Section
Original Research Article

References

Perez-Rodriguez JL, Escriba RCR, Gonzalez GYL, Olmedo JLG, Martenaze -Montero ME. Effect of desiccation on physiological and biochemical indicators associated with the germination and vigor of cryo-preserved seeds of (Nicotiana tabacum L). cv. Sancti Spíritus 96. Tobacco Research Institute, Cuba. In vitro Cell. Dev. Biol. Plant; 2017.

DOI: 10.1007/s11627-017-9857-y

FAO/IPGRI. Gene Bank Standards. Food and Agriculture Organization/ International Plant Genetic Resources Institute, Rome, Italy; 1994.

Roberts EH. Predicting the storage life of seeds. Seed Science & Technology. 1973; 1:499–514.

Michalak M, Plitta-Mchalak BP, Chmielarz P. A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds. Open Life Sci. 2015;10:354–364.

Sastary D, Updahyaya HD, Gowda CLL. Survival of groundnut seeds under different storage conditions International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru. 502 324. Andhra Pradesh, India; 2017.

Roberts EH, Ellis RH. Water and seed survival. Annals of Botany. 1989;63:39– 52.

Walters C, Wheeler L, Standwood PC. Longevity of cryogenically stored seeds. Cryo-Biology. 2004;48:229–244.

Stanwood PC, Bass LN. Seed germplasm conservation using liquid nitrogen. Seed Science & Technology. 1981;9:423–437.

Kartha KK. Cryopreservation of plant cells and organs. CRC Press, Boca Raton, Florida; 1985.

Kartaha KK, Chen THH. Cryopreservation of woody species. In: Cell and Tissue Culure in Forestry, Bonga JM, Durzan DJ. (eds.). Specific principles and Methods: Growth and Development. Dordrecht: Martinus Nijhoff. 1987;2:305 – 319.

Pllipis JR, Oliver MJ, Bartels D. Molecular genetics of desiccation-tolerant systems. In: Desiccation and survival in plant. Drying without dying, Blak M, Prichard HW. Wallingford, UK: CAB International. 2002;319-341.

Stanwood PC. Tolerance of crop seeds to cooling and storage in liquid nitrogen (-196°C). Journal of Seed Technology. 1980;5:26 – 31.

Vertucci CW. Effects of cooling rates on seeds exposed to liquid nitrogen temperature. Plant Physiology. 1989b;90: 1478 – 1485.

Berjak P, Farant JM, Mycock DJ, Pammenter NW. Recalcitrant (homoiohydrous) seeds: The enigma of their desiccation sensitivity. Seed Science & Technology. 1990;18:297–310.

Sastry DK, Rao N, Bramel PJ. Seed drying under controlled environment for long-term conservation of germplasm. Seed Research. 2003;1(2):148–153.

Pammenter NW, Greggains V, Kioko JI, Wesley-Smith J, Berjak P, Finch – Savage WE. Effects of differential drying rates on viabilityretention of recalcitrant seeds of Ekebergia capensis. Seed Science Research. 1998;8:463–471.

Dussert S, Chabrilluber N, Engelmann F, Hamon S. Quantitative estimation of seed desiccation sensitivity using a Quantal Response model: Application to nine species of the genus Coffea L. Seed Science Research. 1999;9:135–144.

Pammenter NW, Berjak P, Wesly-Smith J, Willigen CV. Experimental aspects of drying and recovery. In: Desiccation and survival in Plant: Drying without Dying, Black M, Pritchard HW (eds.). Wallingford, UK: CAB International. 2002;93–110.

Sun WQ. Methods for the study of water relations under desiccation stress In: Black M, Prichard HW. (eds.) Desiccation and Survival in Plant: Drying Without Dying. Wallingford, UK: CAB International. 2002;47–91.

Gomez KA, Gomez AA. Statistical procedures for Agricultural Research, 2nd Ed. John Wiley and Sons Ine. New York; 1984.

Hong TD, Ellis RH, Roberts EH. An intermediate category of seed storage behavior? I. Coffee. Journal of Experimental Botany. 1990a;41:1167– 1174.

Hong TD, Ellis RH, Roberts EH, Tao KL. Low moisture content limits to relations between seed longevity and moisture. Annals of Botany. 1990b;65: 493–504.

Dussert S, Chabrillange N, Rocquelin G, Engelmann F, Lopez M, Human S. Tolerance of coffee (Coffea spp.) seeds to ultra-low temperature exposure in relation to calorimetric properties of tissue water, lipid composition, and cooling procedure. Phisiologia Plantarum. 2001;112:495– 504.

Hor YL, Kim YJ, Ugap A, Chabrillange N, Sinniah UR, Engelmann, Dussert S. Optimal hydration status for cryopreservation of intermediate oily seeds: Citrus as a case study. Annals of Botany. 2005;95:1153–1161.

Makeen AM. Physiological aspects of seed dehydration and cryopreservation of selected citrus Taxsa. Ph. D Thesis, Kembangsaan, University, Malaysia; 2006.

Nautiyal PC. and Joshi, Y.C. Storage of rabid summer groundnut (Arachis hypogaea L.) with calcium chloride for prolonged seed viability and vigour. Tropical Science, (1991). 31:21–26.

Nautiyal PC, Ravindra V, Joshi YC. Moisture stress and subsequent seed viability: Physiological and biochemical basis for viability differences in Spanish groundnut in response to soil moisture stress. Oleagineux. 1991;19:451–459.

Nautiyal PC, Ravindra V. Drying and storage method to prolong seed viability and seedling vigor of Rabi-summer-produced groundnut. Journal of Agronomy and Crop Science. 1996;177:123–128.

Stanwood PC. Dehydration problems associated with the preservation of seed and plant germplasm. In: Membranes, metabolism, and dry organisms, Leopold, A.C. (ed.). Ithaca: Comstock Publishing Associates. 1987a;327–340.

Normah MN. Chin HF, Hor YL. Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Muell. Pertanica. 1986;3:299–303.

Normah MN, Laili NO, Nor-Azza AB, Aliudin R. Effects of desiccation and seed coat on seed germination of four Citrus species Malaya Applied Biology. 1997; 25(1):103–110.

Dickie JB, Pritchard HW. Systematic and evolutionary aspects of desiccation tolerance in seeds. In: Black M, Pritchard HW. (eds.) Desiccation and survival in Plants: Drying without dying. Wallingford, UK: CABI Publishing. 2002;239– 259.

Giambastiani G, Casanoves F. Lipid composition of peanut seeds (Arachis hypogaea L.) obtained under different situations of water availability. Grasas Y Aceites. 2000;51:412–416.

Available:www.ig.csic. es/Revisi/Fas

(Accessed on 23.03.2004)

Vertucci CW, Roos EE. Theoretical basis of protocols for seed storage2. The influence of temperature on optimal moisture level Seed Science Research. 1993;3:201–213.

Vertucci CW. Relationships between thermal transitions and freezing injuries in pea and soybean seeds. Plant Physiology. 1989c;90:1121–1128.

Duong HT. Lunangveririyasaeng V, Shen JL, Ha HT, Pinyopusarerk K. Storage behaviour of Jatropha curcas seeds. Research Centre for Forest Tree Improvement, Forest Science Institute of Vietnam, Tu Liem, Hanoi, Vietnam Journal of Tropical Forest Science. 2013;25(2): 193–199.