Gas Chromatography-Mass Spectrometric Determination of Phenolics and Phthalates in Caro White and Aloe Vera Gel


Published: 2023-12-28

Page: 185-192

Nwoke Ima Bright

Department of Chemistry, Faculty of Natural and Applied Sciences, Ignatius Ajuru University of Education, Rumuolumeni, Port Harcourt, Rivers State, Nigeria.

Batubo Bagshaw Ibifaa

Department of Chemistry, Faculty of Natural and Applied Sciences, Ignatius Ajuru University of Education, Rumuolumeni, Port Harcourt, Rivers State, Nigeria.

Nwoke Prince Lekpoabari *

Department of Chemistry, Faculty of Natural and Applied Sciences, Ignatius Ajuru University of Education, Rumuolumeni, Port Harcourt, Rivers State, Nigeria.

*Author to whom correspondence should be addressed.


Two groups of endocrine-disrupting compounds (phenolics and phthalates) were analyzed in two body creams (Caro white and Aloe Vera Gel) commonly sold in shops within Port Harcourt metropolis using gas chromatography-mass spectrometric (GC-MS) method. The concentrations of twenty-one phenol compounds were identified in both products, with phenol being the most prominent. Caro White Cream exhibited higher phenol concentrations, ranging from 1.31 µg/l to 28.47 µg/l, compared to Aloe Vera Gel Cream, which ranged from 0.37 µg/l to 13.53 µg/l. The study revealed that Caro White Cream contained a total phenol concentration of 129.72 µg/l, while Aloe Vera Gel Cream had 60.47 µg/l. Also, the analysis of phthalates in both creams identified three compounds, with diethyl phthalate being the most prevalent. Caro White Cream exhibited higher phthalate concentrations, ranging from 5.42 µg/l to 13.31 µg/l, compared to Aloe Vera Gel Cream, which ranged from 1.53 µg/l to 5.47 µg/l. The total phthalate concentration in Caro White Cream was 26.20 µg/l, while Aloe Vera Gel Cream had 9.51 µg/l. The combined analysis of phenols and phthalates revealed that Caro White Cream had a total concentration of 155.92 µg/l, indicating potential endocrine-disrupting effects on consumers. In contrast, Aloe Vera Gel Cream had a lower total concentration of 69.98 µg/l. Given the potential health concerns associated with these compounds, consumers are advised by the researchers to review product labels and consider products with simpler, natural ingredients. This study underscores the importance of ingredient transparency and safety in cosmetic products.

Keywords: Endocrine-disrupting compounds, cosmetics, caro white, aloe vera gel, phenols, phthalates

How to Cite

Bright , N. I., Ibifaa , B. B., & Lekpoabari, N. P. (2023). Gas Chromatography-Mass Spectrometric Determination of Phenolics and Phthalates in Caro White and Aloe Vera Gel. Asian Basic and Applied Research Journal, 5(1), 185–192. Retrieved from


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Amato AA, Wheeler HB, Blumberg B. Obesity and endocrine-disrupting chemicals. Endocrine Connections. 2021; 10(2):R87-R105.

Asenuga ER, Olagunju AS. Exposure and associated health risk of endocrine disruption compounds. African Journal of Health, Safety and Environment. 2023; 4(1):57-66.

Guarnotta, V., Azmodei, R., Frasca, F., Aversa, A., & Giordano, C. (2022). Impact of chemical endocrine disruptors and hormone modulators on the endocrine system. International Journal of Molecular Sciences. 2022;23(10):5710.

Lucaccioni L, Trevisani V, Marrozzini L, Bertoncelli N, Predieri B, Lugli L, Iughetti L. Endocrine-disrupting chemicals and their effects during female puberty: A review of current evidence. International Journal of Molecular Sciences. 2020;21(6):2078.

Gore AC, Crews D, Doan LL, La Merrill M, Patisaul, H., & Zota, A. (2014). Introduction to endocrine disrupting chemicals (EDCs). A guide for public interest organizations and policy-makers. 2014;21-22.

Adegoke EO, Rahman MS, Park YJ, Kim YJ, Pang MG. Endocrine-disrupting chemicals and infectious diseases: from endocrine disruption to immunosuppression. International Journal of Molecular Sciences. 2021;22(8):3939.

Dodson RE, Nishioka M, Standley LJ, Perovich LJ, Brody JG, Rudel RA. Endocrine disruptors and asthma-associated chemicals in consumer products. Environmental Health Perspectives. 2012;120(7):935-943.

Helm JS, Nishioka M, Brody JG, Rudel R. A, Dodson RE. Measurement of endocrine disrupting and asthma-associated chemicals in hair products used by Black women. Environmental Research. 2018; 165:448-458.

Berger KP, Kogut KR, Bradman A, She J, Gavin Q, Zahedi R, Harley KG. Personal care product use as a predictor of urinary concentrations of certain phthalates, parabens, and phenols in the HERMOSA study. Journal of Exposure Science & EnvironmentalEpidemiology. 2019;29(1):21-32.

Darbre PD. Human exposure and uptake into human tissues. In Personal Care Products and Human Health Academic Press. 2023;139-190.

Lauretta R, Sansone A, Sansone M, Romanelli F, Appetecchia M. Endocrine disrupting chemicals: Effects on endocrine glands. Frontiers in Endocrinology. 2019;10:178.

Ghosh R, Haque M, Turner PC, Cruz-Cano R, Dallal CM. Racial and sex differences between urinary phthalates and metabolic syndrome among U.S. Adults: NHANES 2005-2014. International Journal of Environmental Research and Public Health. 2021;18(13): 6870. Available:

Branch F, Woodruff TJ, Mitro SD, Zota AR. Vaginal douching and racial/ethnic disparities in phthalates exposures among reproductive-aged women: National Health and Nutrition Examination Survey 2001–2004. Environmental Health. 2015;14: 1-8.

James-Todd TM, Meeker JD, Huang T, Hauser R, Seely EW, Ferguson KK. McElrath TF. Racial and ethnic variations in phthalate metabolite concentration changes across full-term pregnancies. Journal of Exposure Science & Environmental Epidemiology. 2017;27 (2):160-166.

Varshavsky JR, Zota AR, Woodruff TJ. A novel method for calculating potency-weighted cumulative phthalates exposure with implications for identifying racial/ethnic disparities among US reproductive-aged women in NHANES 2001–2012. Environmental Science & Technology, 2016;50(19):10616-10624.

Chinn JJ, Martin IK, Redmond N. Health equity among Black women in the United States. Journal of Women's Health. 2021; 30(2):212-219.

Admon LK, Winkelman TN, Zivin K, Terplan M, Mhyre JM, Dalton VK. Racial and ethnic disparities in the incidence of severe maternal morbidity in the United States, 2012–2015. Obstetrics & Gynecology. 2018;132(5):1158-1166.

Bullard KA, Esperanza R, Sargent JA, Skeith AE, Savitsky LM, Caughey AB. 723: The cost-effectiveness of transvaginal cervical length screening for preterm delivery in nulliparous African American women. American Journal of Obstetrics & Gynecology. 2018;218 (1):S434-S435.

Cabrera SM, Bright GM, Frane JW, Blethen SL, Lee PA. Age of thelarche and menarche in contemporary US females: a cross-sectional analysis. Journal of Pediatric Endocrinology and Metabolism. 2014;27(1-2):47-51.

Callegari LS, Katon JG, Gray KE, Doll K, Pauk S, Lynch KE, Gardella C. Associations between race/ethnicity, uterine fibroids, and minimally invasive hysterectomy in the VA Healthcare System. Women's Health Issues. 2019; 29(1):48-55.

Komorowski AS, Jain T. A review of disparities in access to infertility care and treatment outcomes among Hispanic women. Reproductive Biology and Endocrinology. 2022; 20:1-7.

De Falco, M, Laforgia V. Combined effects of different endocrine-disrupting chemicals (EDCs) on prostate gland. International Journal of Environmental Research and Public Health. 2021;18(18):9772.

Lacouture A, Lafront C, Peillex C, Pelletier M, Audet-Walsh É. Impacts of endocrine-disrupting chemicals on prostate function and cancer. Environmental Research. 2022;204:112085.

Alavian‐Ghavanini A, Rüegg J. Understanding epigenetic effects of endocrine disrupting chemicals: from mechanisms to novel test methods. Basic & Clinical Pharmacology & Toxicology. 2018;122(1):38-45.

Van Cauwenbergh O, Di Serafino A, Tytgat J, Soubry A. Transgenerational epigenetic effects from male exposure to endocrine-disrupting compounds: a systematic review on research in mammals. Clinical Epigenetics. 2020;12(1):1-23.

Kim S, Min HS, Lee WJ, Choe SA. Occupational differences in personal care product use and urinary concentration of endocrine disrupting chemicals by gender. Journal of Exposure Science & Environmental Epidemiology. 2023;33(2):312-318.

Nicolopoulou-Stamati P, Hens L, Sasco AJ. Cosmetics as endocrine disruptors: are they a health risk?. Reviews in Endocrine and Metabolic Disorders. 2015;16:373-383.

Dutta S, Haggerty DK, Rappolee DA. Ruden DM. Phthalate exposure and long-term epigenomic consequences: A review. Frontiers in Genetics, 2020;11:405.

Martín-Pozo L., del Carmen Gómez-Regalado M, Moscoso-Ruiz I, Zafra-Gómez A. Analytical methods for the determination of endocrine disrupting chemicals in cosmetics and personal care products: A review. Talanta. 2021;234:122642.

Dubey P, Reddy SY, Singh V, Shi T, Coltharp M, Clegg D, Dwivedi AK. Association of exposure to phthalate metabolites with sex hormones, obesity, and metabolic syndrome in US women. JAMA Network Ope. 2022;5(9):e2233088-e2233088.

Alaba PAA., Cañete ED., Pantalan BSS, Taguba JMC, Yu LDI, Faller EM. Toxic Effects of Paraben and its Relevance in Cosmetics: A Review. Journal homepage; 2022. Available:www. ijrpr. com ISSN, 2582, 7421

Podlecka D, Gromadzińska J, Mikołajewska K, Fijałkowska B, Stelmach I, Jerzynska, J. (2020). Longitudinal effect of phthalates exposure on allergic diseases in children. Annals of Allergy, Asthma & Immunology. 2020;125(1):84-89.

Bom S Jorge J, Ribeiro HM, Marto JOANA. A step forward on sustainability in the cosmetics industry: A review. Journal of Cleaner Production. 2019;225:270-290.

Abd Gami A, Shukor MY, Khalil KA, Dahalan FA, Khalid A, Ahmad SA. Phenol and its toxicity. Journal of Environmental Microbiology and Toxicology. 2014; 2(1):11-23.

Rivera BN. Assessing Exposures and Simplifying Complex Mixtures of SVOCs for Hazard Characterization using Alternative Model Systems; 2022.

Gardner ST, Wood AT, Lester R, Onkst PE, Burnham N, Perygin DH, Rayburn J. Assessing differences in toxicity and teratogenicity of three phthalates, Diethyl phthalate, Di-n-propyl phthalate, and Di-n-butyl phthalate, using Xenopus laevis embryos. Journal of Toxicology and Environmental Health, Part A. 2016;79(2): 71-82.