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The present study was carried out to evaluate in vitro susceptibility of Clostridium perfringens (C. perfringens) to antimicrobial agents. A total of 22 C. perfringens type A strains, previously isolated from intestinal contents of broiler chickens displaying gross lesions suspected to be necrotic enteritis (NE) reared at different locations in Tiaret province, were tested on a panel of 7 antimicrobial agents using the disk diffusion method. The following antibiotics were used: Amoxicillin-Clavulanic acid (Co-amoxiclav); Penicillin G; Bacitracin; Tetracycline; Clindamycin; Trimethoprim-Sulfamethoxazole (Co-trimoxazole); Erythromycin. Our results showed a widespread resistance to multiple antibiotics including Tetracycline (86.36%), Erythromycin (77.27%), Co-trimoxazole (68.18%) and Co-amoxiclav (50%). However, C. perfringens type A isolates tested demonstrated a low resistance to Clindamycin (36.36%), Bacitracine (36.36%) and Penicillin G (31.81%). We concluded that the highest rate of resistance was showed for commonly used antibiotics in comparison with antibiotics used in specific cases.
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