INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY A PENTACYCLIC TRITERPENES -RICH FRACTION FROM Tabebuia hypoleuca (C. Wright) Urb. LEAVES IN U-251 GLIOBLASTOMA CELLS

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LUZ MARÍA SÁNCHEZ PERERA
ADA IVIS REGALADO VELOZ
ANA LÚCIA TASCA GOIS RUIZ
GIOVANNA BARBARINI LONGATO
GIOVANNA FRANCISCO FIORITO
SIRLENE VALÉRIO TINTI
MARY ANN FOGLIO
JOÃO ERNESTO DE CARVALHO
M. IQBAL CHOUDHARY

Abstract

Background: Tabebuia genera had been reported several pharmacological activities. Cuba has numerous endemic species in this genera, but only our group reported the anti-inflammatory, in vitro and in vivo antiproliferative properties from the specie Tabebuia hypoleuca (C. Wright) Urb. In vitro studies demonstrated that 786-0 (kidney), MCF-7 (breast) and glioma (U-251) were among the cancer cell lines most sensitive to ethyl acetate extract of leaves treatment.

Purpose: The aim of this study was to research the induction of cell cycle arrest and apoptosis by a pentacyclic triterpenes -rich fraction from Tabebuia hypoleuca leaves in U- 251 glioblastoma cells and identify the triterpene structures in this fraction.

Methods: A rich fraction of pentacyclic triterpenes were proceeded for the cell cycle arrest and apoptosis on glioblastoma cells using Annexin V and flow cytometry. The chemical composition was determinate by chromatography and spectroscopic methods.

Results:  Our results showed that a pentacyclic triterpenes –rich fraction had the ability to cause cell arrest in G2/M phase and induce apoptosis. The elucidation of the chemical compounds in this fraction revealed a mixing of oleanolic, ursolic and betulinic acids. This finding about chemical composition in the first report by the genera.

Conclusion: Therefore, this study showed the presence of different pentacyclic triterpenes in T. hypoleuca and it have promising anticancer activity against glioblastoma.

Keywords:
Tabebuia hypoleuca, cell cycle, apoptosis, pentacyclic triterpenes.

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How to Cite
PERERA, L. M., VELOZ, A. I., RUIZ, A. L. T., LONGATO, G., FIORITO, G., TINTI, S., FOGLIO, M., CARVALHO, J. E., & CHOUDHARY, M. (2019). INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY A PENTACYCLIC TRITERPENES -RICH FRACTION FROM Tabebuia hypoleuca (C. Wright) Urb. LEAVES IN U-251 GLIOBLASTOMA CELLS. BIONATURE, 39(2), 70-87. Retrieved from http://globalpresshub.com/index.php/BN/article/view/756
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Short Research Article