COMPARISON OF ETHANOLIC EXTRACT YIELD, pH, POLYPHENOLS AND FLAVONOIDS IN ALGERIAN PROPOLIS COLLECTED FROM VARIOUS GEOGRAPHIC REGIONS
BOUDRA ABDELLATIF *
Department of Animal Health, The Veterinary Institute, Ibn Khaldoun University of Tiaret, 14000, Algeria
BENALLOU BOUABDELLAH
Department of Animal Health, The Veterinary Institute, Ibn Khaldoun University of Tiaret, 14000, Algeria
MELIANI SAMIA *
Faculty of Life Science, Ibn Khaldoun University of Tiaret, 14000, Algeria
HAMDI MOHAMED
Department of Animal Health, The Veterinary Institute, Ibn Khaldoun University of Tiaret, 14000, Algeria
*Author to whom correspondence should be addressed.
Abstract
The present work aims to evaluate the yield, pH, the total polyphenols and flavonoids contents in propolis collected from different ecosystems in Algeria. Ten samples of propolis, issued from different floral and geographical origins, have been collected by beekeepers between March and April 2015 and were classified according to the geographical regions (Est, West, Center and South) the samples were labeled from P1 to P10 and kept in dark at 4°C until use. The determination of polyphenols and total flavonoids contents was carried out in a specialized laboratory in Belgium. In this study, the propolis collected from the southern and western regions had the highest concentrations of polyphenols 99,98; 71,6 and 76,1 Gallic acid/g of propolis. The same result was obtained for flavonoids with 54,4; 38,7 and 42,9 mg Quercetin/g propolis. The higher yield, after ethanolic extraction of the propolis, was obtained from the southern region sample (P10) with a rate of 39,32%. Our study showed that the richest propolis samples of Algeria, in total flavonoid and total polyphenol contents were the ones collected from the southern (P10) and the western region (P6 and P8).
Keywords: Algerian propolis, yield, pH, ethanolic extract