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Response of Sorghum bicolor L. Moench C.S.V. 10 seedlings to different concentrations of sodium fluoride was studied. Characterization and quantification of certain growth characteristics of sodium flouride treated plants indicated decreased fresh weight, dry matter accumulation, shoot and root growth. Though low concentration (0.1mM) of sodium fluoride was found to be optimum for the shoot growth of the seedling as compared with those of the control. Reduction in chlorophyll was also prominent at higher concentrations of sodium fluoride. Similarly starch and protein contents were also decreased considerably with the higher concentrations of sodium fluoride treated Sorghum seedlings.