Associations between ISSR Markers and Quantitative Traits in Bread Wheat Genotypes

Main Article Content

Galal A. R. El-Sherbeny
Mohamed K. Omara
Ashraf A. Farrage
Abdelsabour G. A. Khaled

Abstract

Morphological traits and molecular markers analyses are very important tools for estimation of genetic variability among genotypes. In this study, we combined the phenotypic characterization with the inter-simple sequence repeats (ISSR) technique for the assessment of genetic diversity among 26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P ranged from 25 to 76.92 with an average of 46.97%. The average number of polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level of polymorphism, respectively. It is clear that the highest polymorphism level was obtained in the case of di-nucleotide repeat primers. In this work the polymorphism information content (PIC) values varied from 0.04 (UBC-819) to 0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from 0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers (UBC-811930bp and UBC-833600bp) were identified which may be linked to stem diameter and grain yield per plant traits, respectively.

Keywords:
Triticum aestivum L., molecular markers, grain yield, polymorphism

Article Details

How to Cite
El-Sherbeny, G. A. R., Omara, M. K., Farrage, A. A., & Khaled, A. G. A. (2020). Associations between ISSR Markers and Quantitative Traits in Bread Wheat Genotypes. Asian Journal of Research in Biosciences, 2(1), 1-8. Retrieved from http://globalpresshub.com/index.php/AJORIB/article/view/829
Section
Original Research Article

References

Auvuchanon A. Genetic diversity of wheat cultivars from turkey and U.S. great plains. [PhD Thesis], University of Nebraska, Lincoln, Nebraska; 2010.

Skovmand B, Rajaram S, Ribaut JM, Hede AR. Wheat genetic resources. FAO plant production and protection. Series NO: 30; 2002.
Available:WWW.Fao.Org/DOCREP/006/Y4011e08.htm

Zeven AC, Schavhi R. Groups of bread wheat landraces in the Australian Alps. Euphytica. 1989;41:23-246.

Van Beuningen, LT, Bush RH. Genetic diversity among North American spring wheat cultivars: III. Cluster analysis based on quantitative morphological traits. Crop Sci. 1997;37:981-988.

Maric S, Boleric S, Martinicic J, Petic I, Kozumplic V. Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and coefficient of parentage. Plant Breed. 2004;123:366-369.

Khaled AGA, Hamam KA, Motawea MH, EL-Sherbeny GAR. Genetic analysis and RAPD markers for tissue culture response and some agronomical traits in Egyptian bread wheat. J. genet. Engineering. Biotech. 2013;11:79-86.

Roder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW. Characterisation of PCR-amplified microsatellite loci in wheat. Mol. Gen. Genet. 1995;246:327-333.

Saghai-Maroof MA, Biyashev RB, Yang GP, Zhang Q, Allard RW. Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations and population dynamics. Proc. Natl. Acad. Sci. 1994;91:5466-5470.

Senior ML, Heun M. Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome. 1993;36:884-889.

Wu KS, Tanksley SD. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet. 1993;241: 225-235.

Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS. Study the genetic diversity of wheat landraces from northwest of Iran based on ISSR molecular markers. Int. J. Agric. Biol. 2008;10: 466-468.

Najaphy A, Parchina RA, Farshadfara E. Evaluation of genetic diversity in wheat cultivars and breeding lines using inter simple sequence repeat markers. Biotechnol. Biotec. Eq. 2011;25: 2634-2638.

Khaled AGA, El-Sherbeny GAR, Hadeer SA. SRAP and ISSR molecular markers-trait associations in sorghum genotypes. Assiut J. Agric. Sc. 2019;50(2):72-73.

El-Sherbeny GAR, Khaled AGA, HA Obiadalla-Ali HA, Ahmed AYM. ISSR markers linked to agronomic traits in okra. Int. J. Mod. Agric. 2018;7(1):9-15.

Poresbski SL, Bailey G, Baum RB. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Reporter. 1997;12: 8-15.

Sambrook J, Fritsch RF, Maniatis T. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press ; 1989.

Ghislain M, Zhang D, Hanuman Z, Hijmans R. Marker assisted sampling of the cultivated Andean potato (Solanum phureja) collection using RAPD markers. Genet. Res. Crop Evol. 1999;46:547-555.

Powell W, Morgante M, Andre C, et al. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed. 1996;2(3):225–238.

Nagaoka T, Ogihara Y. Applicability of inter simple sequence repeats polymorphisms in what for use as DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet. 1997; 94:597-602.

Carvalho A, Lima-Brito J, Maçãs B, Guedes-Pinto H. Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochem. Genet. 2009;47:276-294.

Tok D, Senturk-Akfirat F, Sevinc D, Aydin Y, Altinkut-Uncuoglua A. Identification of genetic polymorphism and DNA methyla-tion pattern in wheat (Triticum aestivum L.). Turk. J. Field Crops. 2011;16:157-165.

Diaa A. Characterization of ISSR and SCoT markers and TaWRKY gene expressionin some Egyptian wheat genotypes under drought Stress. Journal of Plant Production Sciences; Suez Canal University. 2019;8(1):31-46.

El-Assal S, Gaber A. Discrimination capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat cultivars. American J. Applied Sci. 2012;9:724-735.

Khaled AGA, Hamam KA. Assocation of molecular markers with phenotypic traits of bread wheat genotypes. Egypt. J. Genet. Cytol. 2015,44:115-130.

Emel S. Evaluation of ISSR markers to assess genetic variability and relationship among winter triticale (X Triticosecale Wittmack) cultivars. Pak. J. Bot. 2010;42:2755-2763.

Song QJ, Fichus EW, Cregan PB. Characterization of trinucleotide SSR motifs in wheat. Theor. Appl. Genet. 2002;104:286-293.

Tatikonda L, Wani SP, Kannan S, Beerelli N, Sreedevi TK, Hoisington DA, Devi P, Varshney RA. AFLP based molecular characterization of an elite germplasm collection of Jatrophacurcas L.: A biofuel plant. Plant Sci. 2009;176:505-513.

Thudi M, Manthena R, Wani SP, Tatikonda L, Hoisington DA, Varshney RA. Analysis of genetic diversity in Pongamia (Pongamiapinnata L. Pierre) using AFLP markers. J. Plant Biochem. Biotechnol. 2010;19:209–216.

Muthusamy S, Kanagarajan S, Ponnusamy S. Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces. Electron. Biotechnol J. 2008; 11:1–10.

Roy JK, Bandopadhyay R, Rustgi S, Balyan HS Gupta PK. Association analysis of agronomically important traits using SSR, SAMPL and AFLP markers in bread wheat. Current Sci. 2006;90:683-689.

Mohammadi A. Majidi-Heravan E, Bihamta MZ, Heidari-Sharifabad H, Hami H. Variation analysis of wheat F3 lines produced by crossing between Azar2 and 87-Zhong291 cultivars using RAPD method in drought stress condition. Iran. J. Genet. Plant Breed. 2010;1:59-64.

El-Rawy MA, Youssef M. Evaluation of drought and heat tolerance in wheat based on seedling traits and molecular analysis. J. Crop Sci. Biotech. 2014;17: 183-189.

Khaled AGA, El-Sherbeny GAR, Haitham MAE. Three SRAP molecular markers linked to yield component traits in wheat. Asian Journal of Research and Review in Agriculture. 2019;1(1):25-35.

Barakat MN, Al-Doss AA, Moustafa KA, Ibrahim E. Morphological and molecular characterization of Saudi wheat genotypes under drought stress. J. Food, Agri. Enviro. 2010;8:220-228.